CURRICULUM VITAE

Personal
Last Name: Cosme Díaz
Name: Karelia
Nationality: Cuban
Address: Ave: 31 No. 18207 e/ 182 y 184 Playa. Havana City
Professional address: Ave. 31 e/158 y 190, 10600, Playa
Telephone: 271 81 64

Educational:
Havana University
(1969-1975) Faculty of Veterinarian
Speciality: Epizootiology
Scientific Category: Auxiliary.
Grade: Doctor in Science Veterinaria
Technologist of Prime Level

Post-grade Courses:
1. Ambient cleaning. Cuba. Julio, 1980.
2. Veterinaries Frontier Services. Epizootiology. Cuba. July, 1980.
3. Geodesy and Cartography. December 1981.
4. International Sanitary Control. Cuba. January 1983.
5. General and Especial Course of Epizootiology. INMV. April, 1983.
6. Transmissible diseases of fauna. Cuba. Nov. 1985.
7. Modern Diagnostic Techniques. FIOCRUZ. Brazil. PNUD. Feb.-March 1988.
8. Molecular Biology. 1989.
9. Seminary of resulted annex 4. Scientific Council of CIGB April 1990
10. Taller Primer Iberoamerican of Gnotobiology. ICLAS. CENPALAB. Nov. 1990.
11. Centro American and Caribbean Course of "Biomodels in Experimentation". Costa Rica. ICLAS. Feb. 1992.
12. International Course of Use and Care of Gnotobiótic Animal. 1992
13. Good Manufacturing Practice. December CIGB 1992-January1993.
14. Seminary of resulted Annex 4 Scientific Council of CIGB. July 1993
15. Laboratory Animal. Ethic, Legislation and regulations. CENPALAB Cuba. 1994.
16. Biology of laboratory animals. CENPALAB Cuba. 1994.
17. Direction management of quality. CIGB, 1994.
18. Facility and Protected System for Animals de lab. CENPALAB. Cuba. Dec 1994 January 1995.
19. Compared medicine, Biosafety and Zoonosys of laboratory animals. CENPALAB. Cuba January to March,1995.
20. System of Laboratory Animal Production. CENPALAB, Cuba. March-April 1995.
21. Biomodels. CENPALAB. Cuba. April-May1995.
22. Economics Aspects for production and use of laboratory animals. CENPALAB Cuba. May-June1995.
23. Nutrition of laboratory animals. CENPALAB. Cuba. June-July 1995.
24. Marketing to Services. National Center of higher studies of Bank. Cuba. March 1996.
25. Basic course of Production. Cuba. April- July1996.
26. Second course of Animal Model. Costa Rica. 1997.
27. Project Integrated Direction. (DIP). High School of Basic Industry. Cuba. April 1999.
28. Course for Auditors interns of the Quality Systems according to ISO 9001-94. February 1999. Cuba. Lloyd s Register.
29. Module I: Introduction of Science for Laboratory Animals. Costa Rica University 2000.
30. Module II: Laboratory Animal Biology. Costa Rica University 2000.
31. Taller Laboratory Animal nutrition. Costa Rica University 2000.
32. Course of "Biotherium" Costa Rica University 2000.
33. "Roll of high management in Norm ISO 9000" 25 de October. 2001.
34. Computation course. February 2002.

Training in and out of the Country:
- Basic techniques of Immunology. FIOCRUZ. Brazil. 1988.
- Hollow Fiber Bioreactor. Butantan Institute. Brazil. 1990.
- Quality Control of bacterial and virals vaccine. INCQS. Brazil. Oct. 1992.
- Training about Biological assays in Animals Models in polyvalent vaccines. National Institute of Quality Control for Health (INCQS), FIOCRUZ. Brazil. September 1995.
- Training in Maintenance and Care of Primates (Chimps). Centre of Primate Research. Netherlands. 2000

PROFESIONAL EXPERIENC:
Academic.
- Parasitology professor. 1968- 1973.
- Assistant professor of Parasitology in clinical laboratory. Havana University 1974.
- Principal professor of basic course "Production in vivo and in vitro again Monoclonal antibodies. Oswald Cruz Foundation. Brazil.1992
- Coordinator and principal professor of theory and practice training the Technicians and professionals of CENPALAB, about tumoral Ascites production in mice. CIGB 1993
- Principal professor to the course "Care and Use of Gnotobiotic animals. Authonoma University. Barcelona, Spain. 1994
- Professor to the course "Application of biological assays in Biotechnological Industry. CIGB 1998
- Principal professor to the course "Use of laboratory animal in Biomedical Science. Animals Models. CIGB Camaguey 1998.
- Professor to the course "Updating of Regulations for quality auditors. CIGB.1998.
- Coordinator and head for training the specialist of the Center for Medicaments and Control (CECMED). Potency test of Hepatitis B recombinant vaccine. CIGB 1999
- Coordinator and principal professor to the course "Guidelines for Care and Use of Laboratory Animal" 2000
- Coordinator and principal professor to the course "Guidelines for Care and Use of Laboratory Animal" 2002

CONFERENCS:
1. Roll of laboratory animal in Biomedical research. Cuba. Oct.1986.
2. Exotic diseases in different animal species in Cuba. Nov. 1986.
3. Immunization system in animal models for antiserums production. Cuba. January 1987.
4. Detection techniques for polyclonal antibodies. Cuba. March 1987.
5. Care and Use of laboratory animal. Cuba. April 1987.
6. Basics aspects of Immunology. Cuba. June. 1987.
7. Adequate comfort for laboratory animal. Facilities. Cuba. Sept. 1987A
8. Characteristics massive of Culture for hybrid cells. Cuba. January 1988
9. Cellular culture in Bioreactors. Cuba. February 1988.
10. Influence of glutamine in grows of hybrids cells. Cuba. April 1988.
11. Large-scale in vitro Hybridoma culture. Cuba. Sept. 1998
12. Clamidia trachomatis. Biology. February. Cuba. 1989.
13. Preparation and obtention of antigen for detection and control of monoclonal antibodies again C. trachomatis. Cuba. April 1989.
14. Diminution or suppression bovine serum in hybrid cells culture. Cuba. June. 1989.
15. Use of hollow fiber reactors. Manufacture facility in cell production. Cuba. July. 1990.
16. Classification of virals diseases (EEC, FDA), in mice and its influence pharmaceutical product quality. Cuba. Feb. 1991.
17. Commercial production of Monoclonal Antibodies. Cuba. Mayo.1991.
18. EEC directresses for Care and Use of laboratory animals. Cuba. January.1992
19. Comparison of ascites production for Monoclonal antibodies in Balb/c-derived
cross-bred mice. May. 1992.
20. Kinetic of Ectromelia virus (Mousepox), transmission and clinical. Cuba. April. 1992.
21. Virus diseases of Group II and I. Characteristics. Cuba. March 1993
22. Animal Models selection for use in the research. Cuba. June. 1993
23. Scale and production of tumoral ascites. Cuba. IX Forum of Science and technique. August 1994
24. Scale and production of CB-HEP.1 Mabs, in Balb/c-derived cross-bred mice. II Meeting ICLAS. Cuba. June.1994
25. Principle of Biosafety. Establishment of rules and regulations. Care of Animal and human health. CENPALAB. February 1995.
26. Biological risk level. Physics, chemical and biologics agents. Cuba. June 1995.
27. Factors affect the experimentation results. Experimental description. Evaluation, discussion and interpretation of experimental results. Cuba. August, 1995.
28. Water. Importance for life (Laboratory Animals). Cuba. Oct. 1995
29. Strategy of GAVAC vaccine. Medellin University. Colombia. 1996
30. Strategy of GAVAC vaccine. Laverlam Empress. Cali. Colombia. 1996
31. Strategy of GAVAC vaccine. Agroavic Empres. Bogotá. Colombia. 1996.
32. Conference about: "Inform of ACCMAL (Centro Americana, Caribbean and Mexican Association for Laboratory Animals) Commission and situation of Laboratory Animals Science in Cuba". II Course of Biomodels. Costa Rica. 1997.
33. Conference: "Utilization of laboratory animals in immunization systems". XVI Pan-American Congress of Veterinarian Science. Santa Cruz, Bolivia. 1998
34. Conference "Employ of laboratory animals in control vaccine for human and veterinarian use. GAVAC". XVII Pan-American Congress of Veterinarian Science. Panama. Sept. 2000

Professorial experience:
1. Guidelines for Maintenance and Care of the laboratory animal. CIGB. Sept.-Nov. 1992.
2. Guidelines for Maintenance and Care of laboratory animal. CIGB. Cuba.
3. Generation and obtention of Monoclonal Antibodies. Large-Scale production. INCQS (Inst. National of Quality Control for Health). Brazil. 1992
4. Iberoamerican course about Care and Use of Laboratory Animal. Animal Models. Use in Biomedicine. CIGB. Sept. 1994
5. Lineaments and Directresses for Maintenance and Care of laboratory animal. CIGB. Sept.-Nov 1994, Cuba.
6. "Care and Use of laboratory Animal. Scale of ascites tumoral production. Bellaterra University. Barcelona. Spain. Septembre.1994.
7. Guidelines for maintenance and care of laboratory animal. CIGB. Sept.-Nov 1995, Cuba.
8. Guidelines for maintenance and care of laboratory animal. CIGB. Sept.-Nov 1996. Cuba.
9. Guidelines for maintenance and care of laboratory animal. Biomodels. April 1997
10. Guidelines for maintenance and care of laboratory animal CIGB. Sept.-Nov 1997. Cuba.
11. Updating regulations for auditors of quality control.1999
12. Coordinator and principal professor of the course about "Guidelines for Use and Care of the laboratory animals. CIGB. 2000
13. Coordinator and principal professor of the course about "Guidelines for Use and Care of the laboratory animals. CIGB. 2002

International events celebrates in Cuba
1. Recommendations for one system of tumoral ascites production in mice employed for antibodies purification. Congress of Biotechnology / Havana 1989. Cartel. April 1989
2. Direct diagnostic of C. trachomatis in patient's samples mediated for monoclonal antibodies. Cartel. April. 1989.
3. Utilization one primer generation hybrid in scale production of tumoral Ascites again Mabs HBsAg. Cartel. Biotechnology / Havana 1990.
4. Ag. preparation. Control for diagnostic use practice of Mabs. Cartel. April. 1992.
5. Large scale Monoclonal Antibody production in ascites using Balb/c and Balb/c-derived cross-bred mice. Conference ICLAS meeting in Centro Americana and Caribbean area. Conference. June1992
6. In vitro Production of Monoclonal Antibodies using a Semi automated Hollow Fiber Bioreactor. Biotechnology / Havana 1992. Cartel.
7. Utilization of the ozone in drink water for laboratory animals. International Symposium of ozone. March 1997.
8. "Employ line dog and rabbits in comparative characterization pharmacokinetic / pharmacodinamic of trombolitics recombinant Streptokinase SK MUT C-42. XII Meeting of Latin-American Association of Animal Production (ALPA) Conventions Palace. 19-23 Nov. Cuba 2001
9. "Application of GMP in monogastrics species employed in biomedical Science. Palace of Conventions. Conference 19-23 Nov. 2001 Cuba X National Meeting of Cuban Association for Animal Production (ACPA)
10. "Employed of deferent's species animals in control and security of Cuban recombinant hepatitis B vaccine. XII Meeting of Latin-American Association for Animal Production (ALPA) Conventions Palace 19-23 Nov. Cuba 2001
11. Determination in vivo of biological activity of Human Factor Transfer. Alternative Technical International Symposium of interferon and Cytokine. CIGB 2001
12. Evaluation of the recombinant Streptokinase as antigen for determination of biological activity of the Human Transfer Factor. International Symposium of interferon and Cytokine. CIGB. 2001
13. Security study of the Human Transfer obtained again no induced cells, administrate by intramuscular source in SD rats. International Symposium of interferon and Cytokine CIGB 2001
14. Quality Assurance in non clinical studies Stimulator Factor of Colonies of Granulocyte human recombinant. International Symposium of interferon and Cytokine CIGB 2001
15. Security, efficacy and effectively of the human recombinant Erythropoietin. International Symposium of interferon and Cytokine. CIGB 2001
16. Evaluations of security of recombinant IL-2 in SD rats. International Symposium of interferon and Cytokine. CIGB 2001
17. Show studies of security and efficacy of the Stimulator Factor of Colonies of Granulocyte, produced in CIGB. 2001
18. Cytokines pattern th 1 /th 2 in animal model of induced Arthritis. International Symposium of interferon and Cytokine. CIGB 2001

INTERNACIONAL EVENTS CELEBRATES OUT CUBA.
1. Scale of reproduction of primer generation line bred OFBALB-F1, obtained in CIGB Biotherium. III Congress of SECAL. Granada. Spain. Nov.1994.
2. Scale of ascites tumoral production in Mabs. again lines Balb/c and hybrids mice. III Congress of SECAL. Granada. Spain. Nov. 1994.
3. Use of laboratory Animal for control to cuban hepatitis B recombinant vaccine. Employ barriers areas. II Course of Biomodels. Costa Rica. 1997.
4. Influence of residual humidity in micro and macro-environment in BALB/c mice. Costa Rica. 1997.
5. Immunologic characterization of HBsAg with component of one combined DPTc-HB vaccine. Costa Rica. 1997.
6. "Obtention, use and care for laboratory animal in Gnotobiotic conditions". II Course about Animal Models in Experimentation. Costa Rica, University 1997.
7. Control in vivo of cuban hepatitis B recombinant vaccine and bovine tick (GAVAC) in barriers zones. XVI Congress PANVET/98 Bolivia
8. Utilization laboratory animals in immunization systems. XVI Congress PANVET/98 Bolivia.
9. GMP application of the monogastrics species employed biomedical Science IV Congress of Society Spain for Science Laboratory Animal (SECAL).. Zaragoza 12-14 Nov. Spain. 2001
10. Employ of different animals species in control and security of cuban hepatitis B recombinant vaccine. IV Congress of Society Spain for Science Laboratory Animal (SECAL). "Zaragoza 12-14 Nov. Spain 2001
11. "Employ dog, rabbits lines in comparative characterization pharmacokinetic / pharmacodinamic of trombolitics recombinant Streptokinase SK MUT C-42. IV Congress of Society Spain for Science Laboratory Animal (SECAL). Zaragoza 12-14 Nov. Spain 2001
12. "Good Manufacturing Practices (GMP) applied to the Care and Use of Laboratory Animals employed in biological assays and quality control. Hepatitis B vaccine and Human Factor Transfer. VIII Symposium FELASA. Aachen, Germany. 2002

National events.
1. Recommendations for establishment one System for high volumes of ascites tumoral production again monoclonal antibodies. IV Seminary scientific of the National Centre of Agriculture Sanity. October 1987.
2. Systems of maintenance for animals in isolators. Second Meeting of Laboratory Animals. Cartel. Marzo.1993
3. Study of deferent adjutants for obtention of major's volumes tumoral ascites again Mabs. Cartel. 1992
4. Scale of reproduction one Biomodel for obtention tumoral ascites. Conference. Nov. 1993
5. Standard Operation Procedure for Maintenance of mice in CIGB Biotherium CIGB. Cartel. 1993
6. Maintenance of animals in protected zones. Nov. 1993.
7. Potency test of Hepatitis B recombinant vaccine. Nov. 1993

Participation in others activities of scientific exchange
- Congress of Biotechnology / Havana 1989
- III seminary international about Interferon. April 1989.
- II Latin-American Congress Biotechnology. Taller of Gnotobiology.
Experimentation Animals for health. August1990
- ICLAS meeting in Centro American and Caribbean area. Nov. 1990
- Congress of Biotechnology / Havana 1992.
- Symposium FIOCRUZ-Inst. Pasteur. Dic. 1992
- International Symposium for advanced systems of the medicaments administration. April 1993.
- II Meeting of ICLAS. Nov. 1993
- Congress of Biotechnology / Havana 1994 (Organizer Committee)
- Congress of Biotechnology / Havana Nov. 95. (Organized Committee)
- Confection of Sanitary Register for Hepatitis B recombinant Vaccine (Heberbiovac HB). CIGB 1997
- Congress of Biotechnology/ Havana. December 1997 (Organized Committee)
- 2nd International Symposium of Applications of the Ozone.1997 Center for Investigations of Ozone. March 1997
- Taller of biological assays. September 1997
- XVI congress Panamerican of Veterinarians Science. August 1998
- Congress of Biotechnology / Havana. November 99
- Congress of Biotechnology / Havana 99. "Production of tumoral ascites again hybrid mice of primer generation. Application of Good Manufacturing Practice.1999
- XII Congress of ICLAS and FELASA conference. Mallorca, Spain. May 1999.
- Latin-American Congress of New Word Primatology. Bogotá 2001

Society and Commission Scientific members.
- Society of Interferon and Biotechnology. 1986
- Society Spain for laboratory Animal Science. SECAL. 1992
- Coordinator of Commission Cuba. Association Centro American, Caribbean and Mexican of Laboratory animal science (ACCMAL). 1992.

PUBLICATIONS:
1. Employ of primer generation hybrids mice OFBALB-F1 in fluid ascites production again Mabs. Vol. 8. Biotecnología Aplicada, 1991.
2. In vitro Production of Monoclonal antibodies using Semi automated Hollow Fiber Bioreactor. Vol. 10 Biotecnología Aplicada 1993.
3. Animals Immunoresponse to the Cuban recombinant yeas-derived anti Hepatitis B viral Surface Antigen (rec. HBsAg.) Vaccine. Vol.10 Biotec. Aplicada, 1993
4. Large Scale Monoclonal Antibody production in ascites using BALB/c and BALB/c-derived cross- bred mice. Vol.10 Biotech. Aplicada. 1993
5. Murine monoclonal antibodies specific for the HBsAg "a". Biotech. Aplicada, Vol.12 No. 2, 1995.
6. Study of the environmental influence in response of antibody for Hepatitis B recombinant vaccine (Heberbiovac HB). Technical inform. February 1996.
7. Application of treatment water with ozone for laboratory animal breeding free of germ pathogen.1998.
8. Methodology for control environmental in Gnotobiotic animals areas. 1999.
9. Monoclonal antibodies production for method of ascites in endogamyc strain BALB/c and hybrid mice OF-1. 1999
10. ALFEBEA a network to harmonize laboratory animal science education and training between Europe and America 1999.
11. Immunologic comportment of the HBsAg with to component one combined DPTc-HB vaccine. 2000.

Received Premiums:
. Obtention and production of antigen for control of Clamidia trachomatis detection for diagnostic use Mabs and direct diagnostic in patients' samples. Scientific Institutional Premium 1987-1988.
. Production of tumoral ascites again monoclonal antibody CB-IFN alfa 2b. Sanitary adequation and initialization installations for animals according to Guide for Use and Care for laboratory animal (NRC, 1985). Scientific Premium Cuba Academic Science in quinquennium 1986-1990.
. Production of monoclonal antibody in bioreactors. Institutional Premium. 1990 1991.
. Establishment of scale in process of production for obtention human Interferon alfa 2b for recombinant source. Scientific National Premium of quinquennium. Cuba Academic Science 1986-1990.
. Establishment of one documentation System for compliments of Good Manufacturing Practice and Laboratory Practice in CIGB and their automatic control. Scientific Institutional Premium 1993.
. Scale of ascites tumoral production again Mabs CB/HEP.1 and Transference of technology for ascites tumoral production at CENPALAB. Scientific Institutional Premium, National Premium of Academic Science of Cuba (ACC). 1994.
. Concurs of Total Quality. Firth Mention. CIGB. Cuba. 1994.
. Hybridoma productor of high levels of specific antibody HBsAg, for purification of the vacunal antigen. Scientific Institutional Premium 1994.
. System of production for high scale of tumoral ascites in protected zones. Development technological transfer. Scientific Institutional Premium, CIGB 1994.
. Establishment conditions of maintenance and care of animals according to GMP. Scientific Institutional Premium 1994-1995.
. Better economic result and Special Premium of Science Technology and Environment Minister 1995-1997. (CITMA). 1997.
. Establishment and introduction of one environmental program for Gnotobiotic animal's areas employed in quality control of biopharmaceuticals products. Scientific Institutional Premium.1998
. Especial Premium CITMA 1998 for the scientific result than more economic impact in this year "Development and introduction of new methods fermentation, immunoafinity and ionic exchange and increase significative the capability of HBsAg production for elaboration of the Cuban vaccine HEBERBIOVAC (HB) in 1996 and 1997.

Scientific-Technical Associations.
. SECAL 1992 Member. Spain.
. ACCMAL 1993 National Coordinator. Costa Rica.
. Pan-American Association of Veterinarian Science.1998. Member. Cuba
. Cuban Society of laboratory animal.1999. Member. Cuba
. Society Brazilian of Animal Welfare.1999. Member. Brazil.

Scientific distinctions.
. Certificate: For your participation in: "Establishment an production process of recombinant human Interferon alfa 2b": Scientific Premium of ACC. 1986-1990. CIGB-ACC.
. Certificate: Institutional Premium. "Production of Mabs in Bioreactors".1990 CIGB.
. Scientific Premium of the ACC. "Scale commercial production and demonstration of the innocuously and effectively the one cuban recombinant hepatitis B vaccine.1992
. Certificate: Scientific Premium of the ACC. "Large scale of ascites tumoral production". 1993
. Certificate: Institutional Premium. "Establishment of integral documentation system for the compliment of GMP and GLP in CIGB and their automatic control".1993.CIGB
. Certificate: Institutional Premium. "Transference ascites tumoral production again monoclonal antibodies". 1994.CIGB
. Certificate: Institutional Premium. "Hybridoma productor high levels of specific antibody (HBsAg) use in vacunal antigen purification".1994.CIGB
. Certificate: Firth mention in concurs of total quality.1994.CIGB
. Diploma: Relevant National Premium. "System of large-scale production of ascites tumoral in protected areas. Development technological transference". 1994. IX Forum of Science and technical.
. Certificate: Institutional Premium and National premium Relevant. "Scale industrial Production of the CB-HEP1 monoclonal antibody for the immunopurification of recombinant antigen of hepatitis B virus employed as vacunal prepared".1995.CIGB
. Certificate: Institutional Premium. "Introduction and establishment the one environmental monitoring program for Gnotobiotics laboratory animals areas employed in quality control of biopharmaceuticals products". 1998 CIGB
. Recognized for magisterial conference and elevated participation. XVI Panamerican Congress Veterinaria Science. Bolivia, 1998
. Institutional Premium. "Establishment and introduction of counter procedures in animal experimentation". Institutional Premium 2000
. Institutional Premium " Establishment and application of GMP at the laboratory animals" 2000
. Institutional Premium "Demonstration quality of the Heberbiovac HB vaccine front other competency vaccine ". 2000
. Institutional Premium "Cuban combined vaccine Diphtheria, Pertussis, Tetanus and Hepatitis B (DPT-HB): Formulation study and evaluation their prolonged stability". 2000
. Institutional Premium "Ingress of the CIGB in Global Alliance Vaccine and Immunization (GAVI) as result the satisfactory evaluation of defended document, in front one Consulter of WHO. 2000
. Scientific Premium of the ACC. Definition of one hepatitis C virus vacunal again results pre-clinical assays. 2000.
. Institutional Premium "Obtention of one Biomodel for immunogenicity determination of the H. influenzae vacunal prepared. 2001.
. Prominent Thesis of Doctor in Science at National level. 2000
. Institutional Premium. "Establishment of one Technology for obtention plasmidic DNA for therapeutic use and human vacunal". 2001
. Institutional Premium "Establishment of one process for obtention of G-CSF. Security and efficacy of evaluation. 2001
. Institutional Premium "Establishment bactericide assays for vacunal prepared evaluation again H. influenzae type b" 2001