jorge Luis

Mario Pablo Estrada García
Director de Investigaciones Agropecuarias

FORMACION PROFESIONAL

1986-1987

Advanced Course in Molecular Biology. Center for Genetic Engineering and Biotechnology. CIGB, Cuba

1987

Research Assistant in the Mammalian Cell Genetics Division. (CIGB, Cuba)

1989

Head of the Cloning Lab from the Mammalian Cell Genetics Division

1992 – 1993

Center Molecular Biology "Severo Ochoa", Universidad Autónoma de Madrid, Spain

1994

Head of the Aquatic Biotechnology Project

2003

Head of the Aquatic Biotechnology Department

2012

Titular Academic of the Academy of Sciences of Cuba

2015

Director of Agricultural Biotechnology Research

Participation in International Meeting and Courses

1987

First Pan- American Teleconference on AIDS, Cuba, (Delegate)

1988

II Jornada Científica. Sanatorio de Santiago de las Vegas, Cuba, Clonaje y expresión de la proteína precursora de la envoltura (GP 160). (Delegate)

1989

III Seminario Cubano e Internacional sobre Interferón. I Seminario Cubano e Internacional sobre Biotecnología. Congreso Iberoamericano sobre Biotecnología, Cuba, Clonaje y expresión del antígeno de superficie del virus de la hepatitis Ben S.cerevisiae. (Delegate)

1990

II Congreso Latinoamericano de Biotecnología, Cuba, 1990, ADN polimerasa del bacteriófago T7 y tiorredoxina de E.coli K-12: clonaje y altos niveles de expresión. (Invited Speaker)

1992

Cardiología ’92. V Congreso Nacional de Cardiología y cirujía cardiovascular. Cuba, Validación experimental de la estreptoquinasa recombinante. (Invited Speaker)

1993

34th Annual Drosophila Research Conference, USA, 1993.

1997

4th International Marine Biotechnology Conference, Italia, . The paradox of growth acceleration in fish and Genetic, biochemical and phenotipic characterization of transgenic tilapia generated by the transfer of a tilapia growth hormone cDNA-containing trangene. (Speaker and Chair person of a session)

2000

International Marine Biotechnology Conference, Tonwsville, Australia. September . Ecological and human-health-effects evaluation of tilapia genetically modified for growth improvement. (Invited Speaker)

2003

Global Biotechnology Forum Latin America And The Caribbean Regional Consultative Meeting. Brasilia, Brazil. 22 - 25 July . (Invited Speaker)

2012

IX Congreso de Ciencias del Mar. MarCuba 2012. Cloning and functional characterization of three novel antimicrobial peptides from tilapia (Oreochromis niloticus)

2013

Speaker on the First International Conference of Fish and Shellfish Immunology, Vigo, Spain, June 25-28, 2013.

Scientific expertise

Construction of genomic libraries in plasmid and phage vectors.

Cloning and expression of foreing genes in E.coli, Yeast and Mammalian Cells

Amplification of DNA fragments (PCR), Southern, Northern and Western blot analysis.

Transgenic research in mammals and aquatic organisms

Molecular adjuvant for veterinary vaccine

Publications

M. P. Estrada, O. Hernández y J. de la Fuente. 1988. Direct expression of mature human growth hormone in E.coli. Interferón y Biotecnología, Vol.5, No. 2, 152-156.

M. P. Estrada, J. R. González, A. Díaz, R. Serrano, P. Rodríguez, L. Hernández, E. Muñoz, M. A. Morín, R. Lezcano, L. Castañeda, Y. Vega, A. González, J. Sánchez, D. Charles-Edouard, J. de la Fuente and L. Herrera. 1992. Pharmacokinetic of 125I-Labelled Recombinant Streptokinase. Biotecnología Aplicada. Vol. 9, No.3, p:281

Alina Aguirre, Eric Perea, Mario P. Estrada, Boris Ramos, José de la Fuente and Fidel O. Castro. 1995. Generation of transgenic mice carrying the E.coli LacZ gene driven by a Drosophila melanogaster patched gene promoter. Short Report Book. Advances in Modern Biotechnology. Vol.3 p: I.17

M. P Estrada, F. Herrera, L. Cabezas, et al. 1998. Fish Performance Studies. (Don MacKinlay, Eds). International Congress on the Biology of the Fish, Towson University, Baltimore, Physiology Section, American Fisheries Society, Pp: 21-27

D. J. Mckenzie, R. Martinezez, A. Morales, J. Acosta, R. Morales, E. W. Taylor, J. F. Steffensen and M. P. Estrada. 2003. Effects of growth hormone transgenesis on metabolic rate, exercise performance and hypoxia tolerance in tilapia hybrids. Journal of Fish Biology. 63, 398–409

Juana Maria Lugo, Alina Rodriguez, Yusmila Helguera, Reynold Morales, Osmany Gonzalez, Jannel Acosta, Vladimir Besada, Aniel Sanchez and Mario Pablo Estrada. 2008. Recombinant novel pituitary adenylate cyclase-activating polypeptide from African catfish (Clarias gariepinus) authenticates its biological function as a growth-promoting factor in low vertebrates. Journal of Endocrinology 197, 1–16

Jannel Acosta, Odalys Ruiz, Yamila Carpio, Reynold Morales, Julio C. Aguila; Jorge Valdés, Eduardo Martinez, Mario Pablo Estrada. 2010. Thermal treatment enhances the stability and biological activity of a truncated tilapia somatotropin contained in Pichia pastoris culture supernatant. Journal of Biotechnology.

Erlinda Fernández, Jorge R. Toledo., Lídice Méndez, Nemecio González, Francisco Parra, Jose´ M. Martín-Alonso, Miladys Limonta, Kosara Sánchez, Ania Cabrales, Mario P. Estrada, Alina Rodríguez-Mallón, Omar Farnós. 2013). Conformational and Thermal Stability Improvements for the Large-Scale Production of Yeast-Derived Rabbit Hemorrhagic Disease Virus-Like Particles as Multipurpose Vaccine. PLoS ONE 8(2): e56417.doi:10.1371/journal.pone.0056417

Rebeca Martínez, Teresa Núñez de Villavicencio-Díaz, Aniel Sánchez, Yassel Ramos, Jesús Noda Ferro, Lázaro Gil González, Milagros Méndez, Elsa Rodríguez, Ernesto Marcos, Belinda Sánchez, YordankMasforrol, HildaGaray, Fernando Albericio, Lisset Hermida, Luis Javier González, Eva Vonasek, Mario P. Estrada, Vladimir Besada. 2016. Comparative proteomic analysis of growth hormone secretagogue A233 treatment of murine macrophage cells J774A.2 indicates it has a role in antiviral innate response. Biochemistry and Biophysics Reports 5(2016)379–387

Patents

  • Method for the isolation and expression of a gene, which codes for streptokinase, nucleotide sequences obtained, recombinant DNA and transformed microorganisms. Application No. 90201930.6-(European Patent Office)
  • Method for the expression of heterologous proteins produced in fused form in E.coli, use thereof, expression vectors and recombinant strains. Application No. 90202108.8- (European Patent Office)
  • Procedimiento para la producción de un antígeno particulado de garrapata en la levadura Pichia pastoris y preparado vacunal obtenido. CU 22326 A1
  • Combinaciones farmacéuticas para el tratamiento del daño tisular debido a falta de irrigación sanguínea arterial. CU 2001/0005
  • Método para la estimulación del crecimiento y resistencia a enfermedades en organismos acuáticos. WO 2003080102 A1
  • Compounds analogous to growth hormone peptide secretagogues and preparations containing them. WO 2007098716 A1
  • Polipéptidos estimuladores del crecimiento para uso en peces y crustáceos. WO 2007009403 A1
  • Neuropéptidos para el cultivo de organismos acuáticos. WO 2007059714 A1
  • Use of pacap for treating viral infections in aquatic organisms. EP 2647369 A1
  • Use of the pacap as a molecular adjuvant for vaccines WO 2013029570 A1
  • Nucleic Acid and Amino Acid Sequences, and Vaccine for the Control of Ectoparasite Infestations in Fish . US 20130280290 A1
  • Vaccine composition for controlling ectoparasite infestations. US 20130273095 A1
  • Amino acid sequences for controlling pathogens. WO 2013044890 A1

Associations and memberships

  • Since 1999. Member of the Steering Committee of the Pan-American Marine Biotechnology Association
  • Member of the International Assessor Committee from the National University “Andrés Bello”, Santiago de Chile, Chile
  • Member of the Scientific Committee of the Center for Genetic Engineering and Biotechnology, Havana, Cuba
  • Editor for Animal Biotechnology of the Journal “Biotecnologia Aplicada”
  • Adjunct President for America of the International Society for Fish and Shellfish Immunology
  • Member of the Scientific Committee of BioCubaFarma
  • Since 2012. Titular Academic of the Academy of Sciences of Cuba
  • Member of the Academic Committee - Master in Science on Biotechnology, CIGB, Havana, Cuba, since 2006
  • Member of the Academic Committee – PhD program of Biological Faculty of Havana University, since 2008
  • Member of the board and head of the technical committee of the project between Marine Harvest and CIGB for the development of a vaccine against sea lice, since 2008 (ongoing project)
  • Head of the technical committee of the project between CENTRO DE INVESTIGACION EN ALIMENTACION Y DESARROLLO (CIAD), Mazatlan, Mexico and CIGB, Havana Cuba, since 2013. “Crecimiento muscular en vertebrados e invertebrados marinos: expresión y función de la miostatina” (ongoing project)
  • Vice President of Pan-American Marine Biotechnology Association (2015)
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