Gerardo Guillen

Gerardo Enrique Guillén Nieto
Director de Investigaciones Biomédicas

TITULO

1986

Título: Master en Ciencias en Química, Universidad de Odessa, URSS.

1995

Doctor en Ciencias Biológicas, Universidad de La Habana, Cuba.

CARGOS

desde 1998

Director de Investigaciones Biomédicas CIGB

Investigador Titular

Biotecnólogo Primer Nivel

POSTGRADOS

1986

Idioma Ruso. Univ. de Odessa. URSS.

1987

Biología Molecular. CIGB.

1987

Idioma Inglés. CIGB.

1987

Genética de Levaduras. CNIC.

1988

Idioma Francés. CNIC.

1989

Sistema de expresión en Baculovirus. Univ. de Helsinki. Finlandia.

1990

Sistema de expresión en Bacillo subtilis. NPHI. Finlandia.

1993

Virología. Univ. de la Habana.

1994

Hepatología. CIGB.

1995-

Tecnología de la obtención de Liposomas. Univ. de la Habana.

1995

Doctor en Ciencias Biológicas, Universidad de La Habana, Cuba.

2001

Curso Avanzado de Vacunología. Fundación Merieux. Francia.

2001

Adiestramiento en "Papel de la Alta Gerencia en las Normas de la Familia ISO 9000". Oficina Nacional de Normalización.

2001

Curso de Inmunología en Enfermedades Infecciosas, impartido por profesores de la Universidad de Harvard. IPK. .

2003

Curso "Nasal Drug Delivery" Management Forum LTD. Londres. Inglaterra

2004

Protección de la información comercial y de negocios. IDICT. Cuba.

2004

Refrescamiento Avanzado en Inmunología, Vaccinología y Biotecnología. OMS. Cuba.

2004

Herramientas para la protección de la información comercial y de negocios. Biomundi. Cuba.

2005

Curso de Interpretación de la Norma ISO 9001-2000. Buro Veritas. La Habana. Septiembre.

2007

Curso Formación de Personal con Base a los Cambios Regulatorios Internacionales. Terra Farma. Mayo 2007

2010

Curso ISO 9000. Principios fundamentales. Interpretación de la Norma ISO 9000:2008. CIGB. La Habana. Julio..

2010

Curso de Bioseguridad en 'áreas biomédicas. La Habana. Noviembre.

2011

International Advanced Immunology Course. May 10-13. ALAI. La Habana.

2012

9th John Humphrey Advanced Immunology Course "Adaptive and Innate Immunity": March 19-21. Fundación del Nuevo Cine Latinoamericano. La Habana.

2015

Curso Pedagogía. Universidad de la Habana. XIV International dengue Course. August 10-21. IPK

CONGRESOS CIENTIFICOS Y CARTELES

1985

  • Participación en más de 180 Congresos Científicos y Talleres Internacionales con la presentación de más de 300 trabajos y más de 30 Conferencias.

CONFERENCIAS Y EVENTOS MÁS RECIENTES


2015

  • APASL, Asian Pacific Association for the Study of the Liver. March 12-15, Istanbul, Turkey..
  • Conferencia: Cuban Biotechnology and CIGB.
  • International Symposiums on Neuroprotection and Peptide Synthesis. April 8-10, Hotel Melia Marina, Varadero, Cuba.
  • V International Meeting CIMAvax-EGF. May 12-13, Palacio de las Convenciones, Havana, Cuba.
  • Conferencia: Virus Like Particles and nucleoparticles as Vaccine Candidates Immunopharmacology – Vaccipharma 2015, Melia Marina Varadero, June 14-19, Matanzas, Cuba .
  • Conferencia: Nanotechnology projects at the Center for Genetic Engineering and Biotechnology. International Seminar on Nanoscience and Nanotechnology, Nano 5, September 14-18, Palacio de las Convenciones, Havana, Cuba .
  • Conferencia: Development of Novel Therapeutic Hepatitis B Vaccine. APASL, Single Topic Conference Focus on the Virus Hepatitis B. Oct. 3-6, Yerevan, Armenia.

2016

  • Assessment of the B. pertussis BPCNIC0311 strain as a producing strain of genetically detoxified toxoid (PTG), Filamentous Hemaglutinin (FHA) 11th International Bordetella Symposium, April 5th-8th, Buenos Aires, Argentina .
  • Conferencia: Cuban Biotechnology and the Center for Genetic Engineering and Biotechnology. Challenges in chronic hepatitis B Conferencia: HeberNasvac Therapeutic Vaccine: from Bench to Clinical Trials. First approval of the marketing authorization dossier of the therapeutic vaccine against chronic Hepatitis B: HeberNasvac. II International Symposium on Viral Hepatitis, Hepatitis 2016, May 19-23. Varadero .
  • Conferencia: HeberNasvac, Novel Therapeutic Vaccine Against Chronic Hepatitis B World Life Science Conference, Nov 1-3. Beijing, China .
  • Conferencia: Nanovaccines: Virus-like Nucleoparticles as vaccine candidates against Dengue. 8th International Workshop on Advanced Material Science and Nanotechnology .
  • Exploring Opportunities for Arbovirus Research Colaboration. Cuba-US., Nov 28-30, Havana, Cuba .
  • Congreso Controlando la Diabetes. Dic 7-9. Varadero .

CURSOS IMPARTIDOS: Más de 50 cursos impartidos


2016

  • Maestría en Virología. Modulo II. Generalidades de Virología y Cultivos Celulares. Unidad Procedimientos Básicos en Virología. 4-15 de Febrero. IPK.
  • Maestría. Procesos Biológicos. CIGB. Modulo Síntesis y aplicaciones de péptidos en la Biotecnología.
  • Maestría Biología Molecular. Diciembre IPK.
  • Maestría. Procesos Biológicos. CIGB. Módulo Virología.

PUBLICACIONES


1. 1,2-Derivatives of acenaphtilene. IV. Characterization of the reaction product from chlorodeoxygenation of 5,6-dichloroacenaphtilen-1,2-dione. 1986. Journal Organicheskaya Jimiya. Academy of Science U.S.S.R. 12:2596-2600.
2. 1,2-Derivatives of acenaphtilene. V. Synthesis of oxide 5,6-dichloroacenaphtilen-1,2-dione. 1986. Journal Organicheskaya Jimiya. Academy of Science U.S.S.R. 10:2204-2208.
3. 1,2-Derivatives of acenaphtilene. VI. Synthesis and characterization of 4-, 3.5-, 5.6-, and 3,5,6-bromoderivatives of 1,2-dihydroacenaphtilen-1,2-diol. 1988. Journal Organicheskaya Jimiya. Academy of Science U.S.S.R. 1:174-181.
4. 1,2-Derivatives of acenaphtilene. X. Characterization of the reaction product from chlorodeoxygenation of 5,6-dibromoacenaphtilen-1,2-dione. 1988. Journal Organicheskaya Jimiya. Academy of Science U.S.S.R. 7:1517-1521.
5. Empleo de marcadores no radioactivos: Fotobiotina y Peroxidasa, en la hibridación de ADN. 1993. Biotecnología Aplicada. 10: 142-145.
6. Comparison of the DNA sequence of nine different genes for the class 1 outer membrane protein from Neisseria meningitidis. 1993. Biotecnologia Aplicada. 10: 108-113.
7. Cloning and expression of the Neisseria meningitidis 5C outer membrane protein. 1995. Acta Biotechnologica. 15(1): 97-106.
8. Enhancement of the immunogenicity of recombinant PM-6 and HBsAg by double experimental vaccines. Pathobiology and Immunobiology of Neisseriaceae. INS. 1994. Memorias de la VIII Conferencia Internacional de Neisserias Patógenas. Octubre 4-9/1992. Cuernavaca. México.
9. Cloning and expression of a fusion protein (PM6-P1.15) from Neisseria meningitidis in Escherichia coli. Pathobiology and Immunobiology of Neisseriaceae. INS. 1994. Memorias de la VIII Conferencia Internacional de Neisserias Patógenas. Octubre 4-9/1992. Cuernavaca. México.
10. Cloning and expression in E.coli of a high molecular weight protein (PM-6) from the N.meningitidis strain B:4:P1.15. Evaluation of the bactericidal activity of antibodies raised against the recombinant protein. Pathobiology and Immunobiology of Neisseriaceae. INS. 1994. Memorias de la VIII Conferencia Internacional de Neisserias Patógenas. Octubre 4-9/1992. Cuernavaca. México.
11. Comparison of DNA sequence of nine class 1 outer membrane protein from different strains of N.meningitidis. Pathobiology and Immunobiology of Neisseriaceae. INS. 1994. Memorias de la VIII Conferencia Internacional de Neisserias Patógenas. Octubre 4-9/1992. Cuernavaca. México.
12. Cloning and expression of the class 1 major outer membrane protein of N.meningitidis stain B:4:P1.15 in E.coli. Pathobiology and Immunobiology of Neisseriaceae. INS. 1994. Memorias de la VIII Conferencia Internacional de Neisserias Patógenas. Octubre 4-9/1992. Cuernavaca. México.
13. Cloning, expresion and characterization of P64K outer membrane protein from N. meningitidis. 1995. Biotecnología Aplicada 12(2): 72
14. Expression in Escherichia coli and immunological characterization of a hybrid class 1-P64k protein from Neisseria meningitidis. 1996. Biotecnología Aplicada 13(4): 271-275
15. Cloning and expression of the porA gene of Neisseria meningitidis strain B:4:P1.15 in Escherichia coli. Preliminary characterisation of the recombinant polypeptide. 1996. Acta Biotechnologica. 16(2-3): 165-173.
16. Molecular analysis of Neisseria meningitidis class 3 outer membrane protein in strains recognized by the Monoclonal antibody CB-Nm.2. 1997. Biotecnología Aplicada 14(1): 23-30
17. Sequence analysis of the structural tbpA gene: protein topology and variable regions within neisserial receptors of transferriniron acquisition. 1997. Microbial Pathogenesis 23: 71-84
18. Induction of immune recognition of self epidermal growth factor-II: characterization of the antibody response and the use of a fusion protein. 1997. Vaccine Research 6(2):91-100
19. Clonación, expresión y características de proteínas quiméricas de Neisseria meningitidis en Escherichia coli. 1997. microbiología e infectología 4 (1): 8-14
20. Expression in Escherichia coli of the lpdA gene, protein sequence analysis and immunological characterization of the P64k protein from Neisseria meningitidis. 1998. Biotechnolo. Appl. Biochem 27: 189-196
21. A novel cancer vaccine composed of human-recombinant epidermal growth factor linked to a carrier protein: Report of a pilot clinical trial. 1998. Annals of Oncology 9:431-435
22. Vacunas contra la hepatitis A y B como componentes de vacunas combinadas. En Bases moleculares para el estudio de las hepatitis virales. Editor: Guillermo J. Padrón. Elfos Scientiae. La Habana, Cuba. 1998. 281-296
23. Characterisation of the lpdA gene from Neisseria meningitidis by polymerase chain reaction, restriction fragment length polymorphism and sequencing. 1999. FEMS Microbiology Letters 174: 191-199
24. Modificación genética de microorganismos. 1999. Biotecnología Aplicada 15(4):
25. Preformulation study of the vaccine candidate P64k against Neisseria meningitidis. 1999. Biotechnolo. Appl. Biochem 29: 113-117
26. New DNA Recombinan Vaccines. 1999. Prodeedings of the International Workshop on Vaccine Development and Production. Vaccine Development: new challenges. Editor Akira Homma. Rio de Janeiro. Finep.
27. P64k Meningococcal Protein as Immunological Carrier for Weak Immunogens. 2000.
Scandinavian Journal of Immunology; 52(2): 113-116
28. Immunogenicity of recombinant class 1 protein from Neisseria meningitidis refolded into phospholipid vesicles and detergent. 2001. Vaccine; 19: 3568-3574

30 Perez A, Dickinson F, Cinza Z, Ruiz A, Serrano T, Sosa J, Gonzalez S, Gutierrez Y, Nazabal C, Gutierrez O, Guzman D, Diaz M, Delgado M, Caballero E, Sardinas G, Alvarez A, Martin A, Guillen G, Silva R. Safety and preliminary immunogenicity of the recombinant outer membrane protein P64k of Neisseria meningitidis in human volunteers. 2001. Biotechnology and Appl. Biochem; 34: 121-125

31 Tozawa K, Broadhurst RW, Raine AR, Fuller C, Alvarez A, Guillen G, Padron G, Perham RN.Solution structure of the lipoyl domain of the chimeric dihydrolipoyl dehydrogenase P64k from Neisseria meningitidis. 2001. European Journal of Biochemistry 268 (18): 4908-4917.

32 Vázquez S, Guzmán MG, Guillen G, Chinea G, Pérez AB, Pupo M, Rodríguez R, Reyes O, Garay HE, Delgado I, García G, Alvarez M. Immune response to synthetic peptides dengue prM protein. 2002. Vaccine 20: 1823-1830

33 L. Hermida, R. Rodríguez, L. Lazo, C. López, G. Márquez, R. Páez, G. Guzmán, C. Suárez, R. Espinosa, J. García , and G. Guillén. A recombinant envelope protein from Dengue virus purified by IMAC is bioequivalent with its immune-affinity chromatography purified counterpart. 2002. J Biotechnology 28;94(2):213-6

34 Guzman MG, Rodriguez R, Rodriguez R, Hermida L, Alvarez M, Lazo L, Mune M, Rosario D, Valdes K, Vazquez S, Martinez R, Serrano T, Paez R, Espinosa R, Pumariega T, Guillen G. Induction of neutralizing antibodies and partial protection from viral challenge in Macaca fascicularis immunized with recombinant dengue 4 virus envelope glycoprotein expressed in Pichia pastoris. 2003. Am J Trop Med Hyg. 69 (2): 619-26

35 Zulueta A., Hermida L., Lazo L., Valdés I., Rodríguez R., López C., Silva R., Rosario D., Martín J., Guzmán MG, Guillén G. The fusion site of envelope fragments from each serotype of dengue virus in the P64k protein, influence some parameters of the resulting chimeric construct 2003. Biochem Biophys Res Commun. 308 (3): 619-26

36 Muné M, Rodriguez R, Ramirez R, Soto Y, Sierra B, Rodriguez-Roche R, Marquez G, García J, Guillén, G Guzmán. Carboxy-terminally truncated Dengue 4 virus envelope glycoprotein expressed in Pichia pastoris induced neutralizing antibodies and resistance to Dengue 4 virus challenge in mice. 2003. Arch Virol 148 (11): 2267-73

37 Aguilar JC, Acosta-Rivero N, Dueñas-Carrera S, Morales Grillo J, Pichardo D, Urquiza D, Guillén G, Muzio V. HCV core protein modulates the immune response against the HBV surface antigen in mice. 2003. Biochem Biophys Res Commun. 310(1): 59-63.

38 Olivia Niebla, Gerardo Guillén, Tamara Menéndez, Anabel Alvarez, Hilda E. Garay,

39 Maite Delgado, Alejandro Martín, Sonia González, Ricardo Silva, Consuelo Nazábal, Dagmara Pichardo, Edelgis Coizeau, Alexis Musacchio. Aportes a la caracterización molecular e inmunológica de porinas de bacterias Gram-negativas y sus regiones variables 2003. Biotecnología Aplicada. 20(1): 60-63.

40 Hermida, L., Rodríguez, R., Lazo L., Silva, R., Zulueta, A., Chinea, G., López, C., Guzmán, M.G. and Guillén, G. A Dengue-2 Envelope fragment inserted within the structure of the P64k meningococcal protein carrier, enables a functional immune response against the virus in mice 2004. Journal of Virological Methods115(1): 41-49.

41 T. Carmenate, L. Canaan, A Alvarez, M Delgado, S Gonzalez, T Menendez, L Rodes, G Guillen. Effect of conjugation methodology on the immunogenicity and protective efficacy of meningococcal group C polysaccharide-P64k protein conjugates. 2004. FEMS Immunology and Medical Microbiology 9;40(3):193-9.

42 Hermida, L., Rodríguez, R., Lazo L, Bernardo L., Silva R., Zulueta A., López C., Martin J., Valdés I., Del Rosario D., Guillén G., Guzmán MG. A fragment of the envelope protein from Dengue-1 virus, fused in two different sites of the meningococcal P64k protein carrier, induces a functional immune response in mice 2004. Biotechnology and Applied Biochemistry. 39: 107-114.

43 JC Aguilar, Y Lobaina, V Muzio, D Garcia, E Penton, E Iglesias, D Pichardo, D Urquiza, D Rodriguez, D Silva, N Petrovsky, G Guillen. Development of a nasal vaccine for chronic hepatitis B infection that uses the ability of hepatitis B core antigen to stimulate a strong Th1response against hepatitis B surface antigen. 2004. Immunology and Cell Biology. 82(5): 539-46.

44 Alvarez A, Martin A, Falcon V, de la Rosa MC, Gonzalez S, Guillen G, Silva R. Membrane association of the P64k dihydrolipoamide dehydrogenase from Neisseria meningitidis. 2004. Biotecnología Aplicada. 21:147-52.

45 T. Carmenate, M. Guirola, A Alvarez, L. Canaan, S Gonzalez, E. Caballero, T Menendez, G Guillen. Memory immune response generated in Cercopithecus aethiops against meningococcal polysaccharide serogroup C conjugated vaccine. 2005. FEMS Immunology and Medical Microbiology. 43(2):133-40.

46 Y. Lobaina, D. Palenzuela, D. Pichardo, V. Muzio, G. Guillén, J.C. Aguilar. Immunological characterization of two hepatitis B core antigen variants and their immunoenhancing effect on co-delivered hepatitis B surface antigen. 2005. Molecular Immunology. 42:289-294.

47 Torrens I, Mendoza O, Batte A, Reyes O, Fernandez LE, Mesa C, Guillen G. Immunotherapy with CTL peptide and VSSP eradicated established human papillomavirus (HPV) type 16 E7-expressing tumors. 2005. Vaccine. 23(50):5768-74

48 Guirola M, Carmenate T, Menendez T, Alvarez A, Gonzalez S, Guillen G. Comparison of three ELISA protocols to measure antibody responses elicited against serogroup C meningococcal polysaccharide in mouse, monkey and human sera. J Microbiol Methods. 2006. 65(1):135-43

49 Maria Guirola, Dioslaida Urquiza, Anabel Alvarez, Leonardo Canaan-Haden, Evelin Caballero and Gerardo Guillen. Immunologic memory response induced by a meningococcal serogroup C conjugate vaccine using the P64k recombinant protein as carrier. FEMS Immunology and Medical Microbiology. 2006 Mar;46(2):169-79.

50 Jorge Aguiar, Julio Fernández, Anselmo Aguilar, Yssel Mendoza, María Vázquez, José Suárez, Jorge Berlanga, Silian Cruz, Gerardo Guillén, Luis Herrera et al. Ubiquitous expression of human SCA2 gene under the regulation of the SCA2 self promoter cause specific Purkinje cell degeneration in transgenic mice. 2006. Neuroscience Letters. 392 (3) 16:202-206.

51 Antonio E. Pérez, Felix O Dickinson, Francisco Banderas, Teresita Serrano, Rafael Llanes, Daymi Guzmán, Pablo Díaz. Anabel Alvarez. Maria Guirola, Evelin caballero, Leonardo Canaan-Haden and Gerardo Guillen. Safety and preliminary immunogenicity of MenC/P64k, a meningococcal serogroup C conjugate vaccine with a new recombinant carrier. 2006. FEMS Immunology and Medical Microbiology. 46 (3): 386-392.

52 Lisset Hermida, Lidice Bernardo, Jorge Martin, Mayling Alvarez, Irina Prado, Carlos Lopez, Beatriz de la C.Sierra, Rafael Martinez, Rosmary Rodríguez, Aida Zulueta, Ana B. Perez, Laura Lazo, Delfina Rosario, Gerardo Guillen, Maria G. Guzman. A recombinant fusion protein containing the Domain III of the dengue-2 envelope protein is immunogenic and protective in nonhuman primates. Lisset Hermida. 2006. Vaccine. 24: 3165-3171.

53 Cibrian, D., Ajamieh H, Berlanga J, Leon OS, Alba JS, Kim MJT, Marchbank T, Boyle JJ, Freyre F, Garcia del Barco D, Lopez Saura P, Guillen G, Ghosh S, Goodlad RA, Plyford RJ. Use of growth hormone releasing peptide-6 for the prevention of multi-organ failure. 2006. Clinical Science. 110 (5): 563-73.

54 Uli L, Castellanos-Serra L, Betancourt L, Dominguez F, Barbera R, Sotolongo F, Guillen G, Pajon R. Outer membrane vesicles of VAMENGOC BC vaccine against serogroup B of Neisseria meningitidis: analysis of protein components by two-dimensional gel electrophoresis and mass spectrometry. 2006. Proteomics. 6 (11): 3389-3399.

55 Y. Cruz, T. Menendez, E. Coizeau, R. Espinosa, L. Cannan, F. Blanco, T. Carmenate, J. Chang, I. Tmargo, J. Cremata, V. Verez, G. Guillén. Enzyme-Linked Immunosorbent Assay for Quantitative Determination of Capsular Polysaccharide in Cultura Supernatants of Streptococcus pneumoniae. 2006. Biotechnology and Applied Biochemistry. 44:101-8

56 Zulueta A. Martín J. Hermida L. Álvarez M. Valdés I. Prado I. Chinea G. Rosario D. Guillén G. Guzmán MG. Amino acid changes in the recombinant Dengue 3 Envelope domain III determine its antigenicity and immunogenicity in mice. 2006. Virus Research. 121(1):65-73.

57 Jorge Berlanga Acosta, William Savigne, Calixto Valdez, Neobalis Franco, Jose S Alba, Amaurys del Rio, Pedro Lopez Saura, Gerardo Guillén, Ernesto Lopez, Luis Herrera, José Fernández Montequín. Epidermal growth factor intralesional infiltrations can prevent amputation in patients with advanced diabetic foot wounds. 2006. Int Wound J. 3(3): 232-9.

58 Enrique Iglesias, Orisley Franch, Yamilka Carrazana, Yadira Lobaina, Daymir García, Jorge Sanchez, José García, Dioslaida Urquiza, Verena Muzio, Gerardo Guillén, Julio C. Aguilar. Influence of the aluminum-based adjuvant on the immune-response to a multi-antigenic formulation. 2006. Viral Immunology. 19(4):712-21.

59 Aguiar J, Fernández J, Aguilar A, Mendoza Y, Vázquez M, Suárez J, Berlanga J, Cruz S, Guillén G, Herrera L, Velázquez L, Santos N, Merino N. Characterization of a new transgenic mouse model of the Spinocerebellar Ataxia type 2 disease. 2006. Biotecnología Aplicada. 23(1):54-9.

60 Laura Lazo, Lisset Hermida, Aída Zulueta, Jorge Sánchez, Carlos López, Ricardo Silva, Gerardo Guillén and María G. Guzmán. A recombinant capsid protein from dengue-2 induces protection in mice against homologous virus. 2007. Vaccine. 25:1064-1070.

61 Valdés I. Hermida L. Zulueta A. Martín J. Silva R. Álvarez M. Guzmán MG. Guillén G. Expression in Pichia pastoris and immunological evaluation of a truncated Dengue envelope protein. 2007. Molecular Biotechnology. 35(1):23-30.

62 Berlanga J, Cibrian D, Guevara L, Dominguez H, Alba JS, Seralena A, Guillen G, Lopez-Mola E, Lopez-Saura P, Rodriguez A, Perez B, Garcia D, Vispo NS.Growth Hormone Releasing Peptide 6 (GHRP6) prevents oxidant cytotoxicity and reduces myocardial necrosis in a model of Acute Myocardial Infarction (AMI). 2007. Clinical Science. 112: 241-250.

63 Aguilar A, Gonzalez CA, Cinza Z, Cabrera J, Veliz G, Moreno SR, Aleman R, Alonso M, Figoroa N, Dias PA, Olivera L, Correa A, Lobaina Y, Delahanty A, Juvier AI, Alonso MI, Lago M, Puble N, David M, Soto RE, Perez MV, Peña ME, Acosta D, Cerna MJ, Mila L, Sanchez R, Hardy E, Guillen G, Muzio VL and Agiar JC. Phase I clinical trial in healthy adults of a nasal vaccine candidate containing recombinant hepatitis B surface and core antigens. 2007. Int J of Inf Dis. 11: 394-401.

64 Enrique Iglesias, Julio C Aguilar, Yamilka Carrazana, Daymir García, Yadira Lobaina1, Verena L Muzio, Gerardo E Guillén, Rafael Thompson, Orisley Franch, Jorge Sánchez, José García, Alejandro Martín, Aniel Sánchez, Emma Brown, Larisa Gorobaya, Dioslaida Urquiza, Otto Cruz. Nueva estrategia para la inducción de respuestas de células CD4+ y CD8+ específicas para una proteína multiepitópica recombinante del VIH-1. 2007. Biotecnología Aplicada. 24(1):79-82.

65 Santiago Dueñas-carrera, Liz Alvarez-lajonchere, Alexis Musacchio, Nelson Acosta-Rivero, Viviana Falcón, Gillian Martínez, Yalena Amador-Cañizares, Ivis Guerra, Julio C Alvarez-Obregón, Angel Pérez, Dagmara Pichardo, Eduardo Martínez, Verena Muzio, Mariela Vázquez, Boris Acevedo, Gerardo Guillén, Karelia Cosme, Marisel Quintana, Pedro López-Saura, Luis Herrera. New alternatives for the development of vaccine preparations: contributions to the knowledge on the interaction of recombinant protein viral antigens with nucleic acids. 2007. Biotecnología Aplicada. 24(3-4):311-314.

66 Jesús Junco, Meter Peschke, Volver Ehrman, Franklin Fuentes, Eddy Bover, Ivan Zuna, Eulogio Pimentel, Maria Castro, Roberto Basulto, Lesvia Calzada, Niurka Arteaga, Yovisleidis Lopez, Hector Hernandez, Osvaldo Reyes, Gerardo Guillen. Immunotherapy of prostate cancer in a murine model using a novel GNRH based vaccine candidate. 2007. Vaccine. 25: 8460-8468.

67 Sheila Cabezasa, Gertrudis Rojas, Alequis Pavon, Mayling Alvarez, Maritza Pupo, Gerardo Guillen and Maria G. Guzman. Selection of phage-displayed human antibody fragments on Dengue virus particles captured by a monoclonal antibody: Application to the four serotypes. 2008. Journal of Virological Methods. 147: 235-243.

68 Lidice Bernardo, Alienys Izquierdo, Irina Prado, Delfina Rosario, Mayling Alvarez, Emidalys Santana, Jorge Castro, Rafael Martínez, Rosmari Rodríguez, Luis Morier, Gerardo Guillén and María G. Guzmán. Primary and secondary infections by Asian and American genotypes of dengue 2 virus in Macaca fascicularis monkeys. 2008. Clinical and Vaccine Immunology. 15 (3): 439-446.

69 Lidice Bernardo, Lisset Hermida, Jorge Martin, Mayling Alvarez, Irina Prado, Carlos López, Rafael Martínez, Rosmari Rodríguez-Roche, Aida Zulueta, Laura Lazo, Delfina Rosario, Gerardo Guillén and María G. Guzmán. Anamnestic Antibody Response After Viral Challenge in Monkeys Immunized with Dengue 2 Recombinant Proteins. 2008. Archives of Virology. 153(5):849-854

70 Aguilar-Betancourt A, González-Delgado CA, Cinza-Estévez Z, Martínez-Cabrera J, Véliz-Ríos G, Alemán-Zaldívar R, Alonso-Martínez MI, Lago-Baños M, Puble-Alvarez N, Delahanty-Fernandez A, Juvier-Madrazo AI, Ortega-León D, Olivera-Ruano L, Correa-Fernández A, Abreu-Reyes D, Soto-Mestre E, Pérez-Pérez MV, Figueroa-Baile N, Pérez LH, Rodríguez-Silva A, Martínez-Díaz E, Guillén-Nieto GE, Muzio-González VL. Safety and immunogenicity of a combined hepatitis B virus-Haemophilus influenzae type B vaccine comprising a synthetic antigen in healthy adults. 2008. Hum Vaccin. Jan-Feb;4(1):54-9.

71 Junco JA, Basalto R, Fuentes F, Bover E, Reyes O, Pimentel E, Calzada L, Castro MD, Arteaga N, López Y, Hernández H, Bringas R, Garay H, Peschke P, Bertot J, Guillén G. Gonadotrophin releasing hormone-based vaccine, an effective candidate for prostate cancer and other hormone-sensitive neoplasms. 2008. Adv Exp Med Biol. 617:581-7.

72 Jorge Berlanga Acosta, Diana Garcia del Barco, Danay Cibrian Vera, William Savigne, Pedro Lopez-Saura, Gerardo Guillen Nieto, and Gregory S. Schultz. The pro-inflammatory environment in recalcitrant diabetic foot wounds. 2008. International Wound Journal. 5(4):530-9.

73 Lidice Bernardo, Alienys Izquierdo, Mayling Alvarez, Delfina Rosario, Irina Prado, Carlos López, Rafael Martínez, Jorge Castro, Emidalys Santana, Lisset Hermida, Gerardo Guillen y María G. Guzmán. Immunogenicity and protective efficacy of a recombinant dengue 1 envelope domain III protein in nonhuman primates. 2008. Antiviral Research 80: 194-9.

74 Jesús A Junco, Roberto Basulto, Franklin Fuentes, Eddy Bover, María D Castro, Osvaldo Reyes, Eulogio Pimentel, Lesvia Calzada, Yovisleidis López, Ricardo Bringas, Niurka Arteaga, Héctor Hernández, Hilda Garay, Luis Agüero, Gerardo Guillén. Inducción de ablación androgénica e inhibición de tumores hormonosensibles de próstata mediante inmunización con el péptido sintético GnRHm1-TT adyuvado con VSSP. 2008. Biotecnología Aplicada. 25(2): 171-6.

75 Izquierdo A, Bernardo L, Martin J, Santana E, Hermida L, Guillén G, Guzmán MG. Serotype-specificity of recombinant fusion proteins containing domain III of dengue virus. 2008. Virus Res 138(1-2):135-8.

76 Carlos López, Laura Lazo, Lázaro Gil, Lisset Hermida, Gerardo Guillén, Guadalupe Guzmán, Susana Vázquez, Lídice Bernardo, Aída Zulueta, Jorge Martín, Iris Valdés, Jorge Sánchez, Aracelys Blanco, Yaremis Romero, Ivón Menéndez, María C de la Rosa, Gabriel Márquez, Ricardo Silva, Viviana Falcón, Manuel Selman-Housein. New evidence on the virus capsid as a vaccine candidate against the Dengue 2 virus without the induction of neutralizing antibodies”. Report. 2008. Biotecnología Aplicada Enr.-Mar, 25(1).

77 T. Menéndez and G. Guillen. Recent Advances in the Pathogenic Neisseria Research. 2008. Biotecnología Aplicada. 25 (3)

78 Viviana Falcón, Santiago Dueñas, Ivón Menéndez, Maria Cristina de la Rosa, Juan Morales, José Ángel Silva, Emilio Felino Acosta, Félix Álvarez, Jesús Seoane, Zurina Cinza, Glay Chinea, Jorge V. Gavilondo, Celia Fernández, Dionne Casillas, Verena Muzio, Eduardo Pentón, Hugo Nodarse, Liz Álvarez-Lajonchere, Gillian Martínez, Ivis Guerra, Hedí. E González, Bienvenido Gra, Enríquez Arús, Eduardo Vidal, Julio César Hernández, Mineko Shibayama, Edel Torres, José Luna-Muñoz, Magdalena Miranda-Sánchez, Juan Kouri, Víctor Tsutsumi, Gerardo Guilén. Aportes al estudio de los mecanismos patogénicos del VHC y sus implicaciones en la comprensión de la enfermedad. 2008. Biotecnología Aplicada Oct.-Dic., 25(4): 1-2.

79 Alicia Santos, Ania Cabrales, Haydee Gerónimo, Osvaldo Reyes, Yunier Rodríguez, Hilda E. Garay, Celia A. Arrieta, Ricardo Silva and Gerardo Guillén. Identification of an Interleukin-15 Antagonist peptide that binds to IL-15R alpha. 2008. Biotecnología Aplicada Oct.-Dic. 25(4): 320-4.

80 Lobaina, Y, García, D, Iglesias, E, Muzio, V, Rodríguez, D, Gorovaya, L, Pentón, E, Guillen, G, Aguilar, J.C. Comparison of the immune response induced in mice by five commercial vaccines based on recombinant HBsAg from different sources, implications on their therapeutic use. 2008. Biotecnología Aplicada Oct.-Dic. 25(4): 325-31.

81 Maité Delgado, Daniel Yero, Olivia Niebla, Gretel Sardiñas, Sonia González, Evelín Caballero, Yanet Climent, Darién García, Karem Cobas, Isbel Vivar, Yusleydis Pérez, Laritza Gorovaya, Lázaro H Betancourt, Yasser Perera, Lila Castellanos, Gerardo Guillén. Analysis of the genetic variability and immunological properties of the NlpB antigen, a novel protein identified in Neisseria meningitides. 2008. Biotecnología Aplicada 26(1): 84-87.

82 Laura Lazo, Aída Zulueta, Lisset Hermida, Aracelys Blanco, Jorge Sánchez, Iris Valdés, Lázaro Gil, Carlos López, Yaremis Romero, María G. Guzmán and Gerardo Guillén. Dengue-4 envelope domain III fused twice within the meningococcal P64k protein carrier induces partial protection in mice. 2009. Biotechnol Appl Biochem. 52(Pt 4):265-71.

83 Lazo L, Gil L, Zulueta A, Valdés I, Soria Y, Romero Y, Guillén G, Hermida L. Comparative immunogenicity and protective capacity of two Dengue-4 vaccine candidates based on P64k-envelope domain III. 2009. Biotecnología Aplicada 26(4):333-37

84 Jeovanis Gil, Lázaro H. Betancourt, Gretel Sardiñas, Daniel Yero, Olivia Niebla, Maité Delgado, Darien García, Rolando Pajón, Aniel Sánchez, Luis J. González, Gabriel Padrón, Concepción Campa, Franklin Sotolongo, Ramón Barberá, Gerardo Guillén, Luis Herrera and Vladimir Besada. Proteomic study via a non-gel based approach of meningococcal outer membrane vesicle vaccine obtained from strain CU385: a road map for discovery new antigens. 2009. Human Vaccines 5(5): 347-356.

85 Lazaro Gil, Carlos Lopez, Aracelys Blanco, Laura Lazo, Jorge Martín, Iris Valdes, Yaremis Romero, Yassel Figueroa, Gerardo Guillen, Lisset Hermida. The cellular immune response plays a crucial role in the dengue virus encephalitis murine model. 2009. Viral Immunology, 22(1):23-30.

86 Iris Valdés, Lisset Hermida, Jorge Martín, Tamara Menéndez, Lázaro Gil, Laura Lazo, Jorge Castro, Olivia Niebla, Carlos López, Lídice Bernardo, Jorge Sánchez, Yaremis Romero, Rafael Martínez, María G. Guzmán, Gerardo Guillén. Immunological evaluation in nonhuman primates of formulations based on the chimeric protein p64k-domain III of dengue 2 and two components of Neisseria meningitidis. 2009. Vaccine. 27: 995-1001.

87 López C, Gil L, Lazo L, Menéndez I, Marcos E, Sánchez J, Valdés I, Falcón V, de la Rosa MC, Márquez G, Guillén G, Hermida L. In vitro assembly of nucleocapsid-like particles from purified recombinant capsid protein of dengue-2 virus. 2009. Archives of Virology 154(4):695-8.

88 Martín, Jorge; Hermida, Lisset; Castro, Jorge; Lazo, Laura; Martinez, Rafael; Gil, Lázaro; Romero, Yaremis; Puente, Pedro; Zaragoza, Santiago; Cosme, Karelia; Guzmán, María; Cardosa, Jane; Guillén, Gerardo. Viremia and antibody response in green monkeys (Chlorocebus aethiops sabaeus) infected with dengue 2 virus: a potential model for vaccine testing. 2009. Microbiology and Immunology. 59(6):579-83.

89 Jorge Martín, Lisset Hermida, Jorge Castro, Yaremis Romero, Jane Cardosa, Gerardo Guillén. Viremia and the Magnitude of the Immune Response upon Infection of Green Monkeys with Dengue Virus Type 2 Are Strain-Dependent. 2009. Current Microbiology 59:579-83.

90 Cabezas S, Bernardo L, Pavon A, Pupo M, Castellanos Y, Rojas G, Alvarez M, Guillen G, Guzmán MG. Phage-displayed antibody fragments recognizing dengue 3 and dengue 4 viruses as tools for viral serotyping in sera from infected individuals. 2009. Archives of Virology 154:1035-45.

91 Gil. L, Alonso. R, López. C, Blanco. A, Romero. Y, Guillén. G, Hermida. L. A CFSE-based assay of proliferative response of lymphocytes to stimulation with dengue viruses. 2009. Acta virologica 53(3):147-52.

92 Lázaro Gil, Carlos López, Laura Lazo, Iris Valdés, Ruby Alonso, Ernesto Marcos, A. Gambe, Jorge Martín, Yaremis Romero, María G. Guzmán, Gerardo Guillén, Lisset Hermida. Recombinant nucleocapsid-like particles from dengue-2 virus induce protective CD4+ and CD8+ cells against viral encephalitis in mice. 2009. International Immunology. 21(10):1175-83.

93 Jorge Berlanga-Acosta; Jorge Gavilondo-Cowley; Pedro López-Saura; Tania González-López; María D Castro-Santana; Ernesto López-Mola; Gerardo Guillén Nieto; Luis Herrera-Martinez. Epidermal Growth Factor (EGF) in clinical practice- A review of its biological actions, clinical indications and safety implications. 2009. International Wound Journal 6(5): 331-46.

94 Iris Valdés, Lídice Bernardo, Lázaro Gil, Alekis Pavón, Laura Lazo, Carlos López, Yaremis Romero, Ivon Menendez, Viviana Falcon, Lázaro Betancourt, Jorge Martín, Glay Chinea, Ricardo Silva, María G. Guzmán, Gerardo Guillén, Lisset Hermida. A novel fusion protein domain III-capsid from dengue-2, in a highly aggregated form, induces functional immune response and protection in mice. 2009. Virology 25;394(2):249-58.

95 Bernardo L, Fleitas O, Pavón A, Hermida L, Guillén G, Guzman MG. Antibodies induced by dengue 1 and dengue 2 envelope domain III recombinant proteins in monkeys neutralize strains of different genotypes. 2009. Clin Vaccine Immunol. 16(12):1829-31.

96 Iris Valdés, Jorge Sanchez, Laura Lazo, Jorge Martín, Yaremis Romero, Yadira Rodriguez, Gerardo Guillen, Lisset Hermida. Evaluation of different formulations of a dengue-2 chimeric protein and outer membrane vesicles from Neisseria meningitidis in mice. 2009. Biotecnología Aplicada 26(3):209-213.

97 Iglesias, E and Guillén, G. Report of the V Conference of IAS on HIV pathogenesis, treatment and prevention of HIV. 2009. Biotecnología Aplicada 26: 251-254

98 Tamara Menéndez, Tania Carmenate, Yoelys Cruz-Leal, Edelgis Coizeau, Evelyn Caballero, Daniel Bello, María Guirola, Anabel Álvarez and Gerardo Guillén. Purified capsular polysaccharide of Neisseria meningitidis serogroup A as immune potentiator for antibody production. 2010. Current Microbiology 60(2):79-84

99 Iris Valdés, Lisset Hermida, Lázaro Gil, Laura Lazo, Jorge Castro, Jorge Martín, Lídice Bernardo, Carlos López, Olivia Niebla, Tamara Menéndez, Yaremis Romero, Jorge Sánchez, Guadalupe Guzman, Gerardo Guillen. Heterologous prime-boost strategy in nonhuman primates combining the infective dengue virus and a recombinant protein in a formulation suitable for human use. 2010. International Journal of Infectious Diseases. 14(5): 377-83.

100 Guzman MG, Hermida L, Bernardo L, Ramirez R, Guillén G. Domain III of the envelope protein as a dengue vaccine target. 2010. Expert Review of Vaccines 9(2): 137-147.

101 Lazo L., Gil L., Lopez C., Valdes I., Marcos E., Alvarez M., Blanco A., Romero Y., Falcon V., Guzman MG., Guillen G., Hermida L. Nucleocapsid-like particles of dengue-2 virus enhance the immune response against a recombinant protein of dengue-4 virus. 2010. Arch Virol. 155:1587–1595.

102 Guillén G, Aguilar JC, Dueñas S, Hermida L, Guzmán MG, Penton E, Iglesias E, Junco J, Torrens I, Lobaina Y, Muzio V, L. Herrera. Virus-Like Particles as vaccine antigens and adjuvants: application to chronic disease, cancer immunotherapy and infectious disease preventive strategies. 2010. Procedia in Vaccinology 2: 128–133.

103 Lazo L., Gil L., Zulueta A., Valdés I., Soria Y., Romero Y., Guillén G., Hermida L. Immunological evaluation in mice of two Dengue-4 vaccine candidates based on P64k-envelope domain III.2010. Biotecnología Aplicada 26(4).

104 Vallespi MG, Fernandez JR, Torrens I, Garcia I, Garay H, Mendoza O, Granadillo M, Falcon V, Acevedo B, Ubieta R, Guillen GE, Reyes O. Identification of a novel antitumor peptide based on the screening of an Ala-library derived from the LALF(32-51) region. 2010. J Pept Sci. 16(1):40-7.

105 Tamara Menéndez, Nelson F Santiago-Vispo, Yoelys Cruz-Leal, Edelgis Coizeau, Hilda Garay, Osvaldo Reyes, Yaima Batista, Karem Cobas, Tania Carmenate, Glay Chinea, Gerardo Guillén. Identification and characterization of phage-displayed peptide mimetics of Neisseria meningitidis serogroup B capsular polysaccharide. 2011. International Journal of Medical Microbiology 301(1):16-25.

106 Garay, Hilda; Menendez, Tamara; Cruz-Leal, Yoelys; Coizeau, Edelgis; Noda, Jesus; Morera, Vivian; Guillen, Gerardo; Albericio, Fernando; Reyes, Osvaldo. Study of various presentation forms for a peptide mimetic of Neisseria meningitidis serogroup B capsular polysaccharide. 2011. Bioconjugate Chemistry 22(1):33-41.

107 Pentón-Rol G, Martínez-Sánchez G, Cervantes-Llanos M, Lagumersindez-Denis N, Acosta-Medina EF, Falcón-Cama V, Alonso-Ramírez R, Valenzuela-Silva C, Rodríguez-Jiménez E, Llópiz-Arzuaga A, Marín-Prida J, López-Saura PA, Guillén-Nieto GE, Pentón-Arias E. C-Phycocyanin ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells. 2011. Int Immunopharmacol. Jan;11(1):29-38. Epub 2010 Oct 23.

108 Iris Valdes Prado; Lázaro Gil González; Yaremis Romero Fernández; Jorge Castro Velazco; Pedro Puente Pérez; Laura Lazo Vazquez; Ernesto Marcos Lopez; Guadalupe Guzmán; Gerardo Enrique Guillen Nieto; Lisset Hermida Cruz. The chimeric protein domain III-capsid of DEN-2 successfully boosts neutralizing antibodies generated in monkeys upon infection with DEN-2 virus. 2011. Clinical and Vaccine Immunology. 18(3):455-9.

109 Jorge Berlanga-Acosta, Jorge Gavilondo-Cowley, Diana García del Barco-Herrera, Jorge Martín-Machado and Gerardo Guillen Nieto. Epidermal Growth Factor (EGF) and Platelet-Derived Growth Factor (PDGF) as Tissue Healing Agents: Clarifying Concerns about their Possible Role in Malignant Transformation and Tumor Progression. 2011. J Carcinogene Mutagene 2 (1): 1000115 open access journal http://dx.doi.org/4172/2157-2518.1000115

110 Alicia Santos Savio, Osvaldo Reyes Acosta, Haydee Gerónimo Pérez, Yunier Rodríguez Álvarez, Araceli Chico, Hilda Garay Pérez, Miriam Ojeda Ojeda, Celia Aurora Arrieta Aguero, Miguel Estévez, Gerardo Guillen Nieto. Enhancement of the inhibitory effect of an IL-15 antagonist peptide by alanine scanning. 2011. J Pept Sci. 18(1):25-9.

111 Bernardo L, Pavón A, Hermida L, Gil L, Valdés I, Cabezas S, Linares R, Alvarez M, Silva R, Guillén G, Nagy E, Schlick P, Guzmán MG. The two component adjuvant IC31(®) potentiates the protective immunity induced by a dengue 2 recombinant fusion protein in mice. 2011. Vaccine. Jun 6; 29(25):4256-63.

112 Pentón-Rol G, Marín-Prida J, Pardo-Andreu G, Martínez-Sánchez G, Acosta-Medina EF, Valdivia-Acosta A, Lagumersindez-Denis N, Rodríguez-Jiménez E, Llópiz-Arzuaga A, López-Saura PA, Guillén-Nieto G, Pentón-Arias E. C-Phycocyanin is neuroprotective against global cerebral ischemia/reperfusion injury in gerbils. 2011. Brain Res Bull. 86(1-2):42-52.

113 M.G.Guzman, G. Guillen, E.M. Perez. Vacuna Dengue. Investigaciones en Cuba dirigidas a la búsqueda de un candidato vacunal efectivo. In: Vacunas Cuba 1959-2008. Editor: Francisco Rojas Ochoa. Editorial Ciencias Médicas. 2011. 20: 191-210.

114 G. Guillén, A. Aguilera, V. Muzio, J.C. Aguilar. Los Nuevos Sueños…. In: Vacunas Cuba 1959-2008. Editor: Francisco Rojas Ochoa. Editorial Ciencias Médicas. 2011. 22: 229-234.

115 J.A. Junco, F. Fuentes, R. Basulto, E. Bover, M.D. Castro, E. Pimentel, O. Reyes, R. Bringas, L. Calzada, Y. López, N. Arteaga, A. Rodríguez, H. Garay, R. Rodríguez, L. González-Quiza, L. Fong and G.E. Guillén. Prostate Cancer Immunotherapy – Strategy with a Synthetic GnRH Based Vaccine Candidate. In: Advancements in Tumor Immunotherapy and Cancer Vaccines. Editor: Hilal Arnouk. InTech. 2012. 63-82.

116 Dania Marcia Vazquez Blomquist; Yssel Mendoza Mari; Maria Elena Ochagavia Roque; Jamilet Miranda Navarro; Ricardo Bringas Perez; Daniel Octavio Palenzuela Gardon; Lidia Ines Novoa; Marcelo Nazabal Galvez; Alberto Cintado Benitez; Isabel Alicia Guillen Perez; José Suárez Alba; Diana Garcia del Barco Herrera; Karelia Cosme Diaz; julio fernandez; Ernesto Lopez Mola; Gerardo Enrique Guillen Nieto; Jorge Berlanga Acosta. Growth Hormone Releasing Peptide 6 (GHRP6) reduces liver fibrosis in CCL4 chronically intoxicated rats. 2012. Biotecnología Aplicada 29 (9) (In press).

117 D. Limonta, V. Falcón, G. Torres, V. Capo, I. Menéndez, D. Rosario, Y. Castellanos, M. Alvarez, R. Rodríguez-Roche, M. C. de la Rosa, A. Pavón, L. López, K. González, G. Guillén, J. Díaz and M. G. Guzmán. Dengue virus identification by transmission electron microscopy and molecular methods in fatal dengue hemorrhagic fever. 2012. Infection 40(1) (In press).

118 Alienys Izquierdo, Iris Valdés, Lázaro Gil, Lisset Hermida, Sheila Gutiérrez, Angélica García, Lidice Bernardo, Alekis Pavón, Gerardo Guillén, María G Guzmán. Serotype specificity of recombinant fusion protein containing domain III and capsid protein of dengue virus 2. 2012. Antiviral Research 95(1):1-8.

119 Lázaro Gil, Lidice Bernardo, Alequis Pavón, Alienys Izquierdo, Iris Valdés, Laura Lazo, Ernesto Marcos, Yaremis Romero, María G. Guzmán, Gerardo Guillen and Lisset Hermida. Recombinant nucleocapsid-like particles from dengue-2 induce functional serotype-specific cell-mediated immunity in mice. 2012. Journal of General Virology 93: 1204-14.

120 Maryline Bourgine, Sarah Dion, Ophelie Godon, Gerardo Guillen, Marie-Louise Michel, Julio Cesar Aguilar. Optimization of immune responses induced by therapeutic vaccination with cross-reactive antigens in humanized hepatitis B surface antigen transgenic mouse model. 2012. Virology 430: 10-19.

121 Ana Cecilia Machado Diaz, Araceli Chico Capote, Celia A. Arrieta Aguero, Yunier Rodriguez Alvarez, Diana Garcia del Barco Herrera, Miguel Estevez del Toro, Gerardo E. Guillen Nieto, and Alicia Santos Sabio. Proinflammatory Soluble Interleukine-15 Receptor alpha is increased in Rheumathoid Arthritis. 2012. Arthritis (In press).

122 Laura Lazo, Lázaro Gil, Carlos López, Iris Valdés, Aracelys Blanco, Alequis Pavón, Yaremis Romero, María G Guzmán, Gerardo Guillén, Lisset Hermida. A vaccine formulation consisting of nucleocapsid-like particles from Dengue-2 and the fusion protein P64k-domain III from Dengue-1 induces a protective immune response against the homologous serotypes in mice. 2012. Acta Tropica 06 (In press)

123 Lisset Hermida, Iris Valdés, Lázaro Gil, Lídice Bernardo, Laura Lazo, Yaremis Romero, María G. Guzmán & Gerardo Guillén. The serogroup A capsular polysaccharide from Neisseria meningitidis enhances the cell-mediated immunity and the protective capacity induced by a dengue fusion protein in mice. 2012. Archives of Virology 157(5):987-91.

124 Ania Cabrales, Jeovanis Gil, Eduardo Fernández, Carmen Valenzuela, Francisco Hernández, Idrián García, Ariadna Hernández, Vladimir Besada, Osvaldo Reyes, Gabriel Padrón, Jorge Berlanga, Gerardo Guillén, Luis Javier González. Pharmacokinetic study of Growth Hormone-Releasing Peptide 6 (GHRP-6) in nine male healthy volunteers European Journal of Pharmaceutical Sciences. 2012. European Journal of Pharmaceutical Sciences (In press)

125 Franklin Fuentes Aguilara, Jesús Junco Barrancoa, Eddy Bover Fuentesa, Lesvia Calzada Aguileraa, Yovisleydis Lopez Sáeza, María Dolores Castro Santanaa, Eulogio Pimentel Vázqueza, Roberto Basulto Bakera, Osvaldo Reyes Acostab, Hilda Garay Pérezb, Gerardo Guillén Nietob. Very Small Size Proteoliposomes (VSSP) and Montanide combination enhance the humoral immuno response in a GnRH based vaccine directed to prostate cancer. 2012. Vaccine 30: 6595-9.

126 Isabel A Guillén, Hanlet Camacho, María E Fernández, Daniel O Palenzuela, Lincidio Pérez, María E Ochagavia, Julio A Ancizar, Ángela D Tuero, Laritza Gorovaya, Osmani Mendoza, Tamara Díaz, Julio R Fernández, Juan Roca, Karelia Cosme, Gerardo E Guillén-Nieto, Luis Herrera, Jorge Berlanga, Lidia I Novoa. Effect of human epidermal growth factor on the tumor cell line A431: in vivo analysis of tumor growth inhibition and gene expression. 2012. Biotecnología Aplicada. 29(3): 165-171.

127 Jorge Berlanga-Acosta, Ernesto López-Mola, Marianela García-Siverio, Gerardo Guillén-Nieto, Pedro López-Saura, Calixto Valdez-Pérez, Isabel Puentes-Madera, William Savigne-Gutiérrez, Héctor Álvarez-Duarte, Norberto Miranda-Espinosa, Ana J Mir-Benítez, Diana García del Barco, Yssel Mendoza-Mari, María D Martínez-Espina, Ariana García-Ojalvo, Nelvys Subiros-Martínez,Luis Herrera-Martínez. Detrimental impact of acute and chronic glucose burden in wound-healing cells: fibroblasts, myofibroblasts and vascular precursor cells. 2012. Biotecnología Aplicada. 29(4): 208-17.

128 Perez-Riverol Y, Hermjakob H, Kohlbacher O, Martens L, Creasy D, Cox J, Leprevost F, Shan BP, Pérez-Nueno VI, Blazejczyk M, Punta M, Vierlinger K, Valiente P, Leon K, Chinea G, Guirola O, Bringas R, Cabrera G, Guillen G, Padron G, Gonzalez LJ, Besada V. Computational Proteomics Pitfalls and Challenges: HavanaBioinfo 2012 Workshop Report. 2013. Journal of Proteomics 13: S1874-3919

129 Marcos E, Gil L, Lazo L, Izquierdo A, Brown E, Suzarte E, Valdés I, García A, Méndez L, Guzmán MG, Guillén G, Hermida L. Purified and highly aggregated chimeric protein DIIIC-2 induces a functional immune response in mice against dengue 2 virus. 2013. Arch Virol 158(1):225-30.

130 Guillén G, Aguilar JC, Dueñas S, Hermida L, Iglesias E, Penton E, Lobaina Y, Lopez M, Musacchio A, Falcon V, Alvarez L, Martinez G, Gil L, Valdes I, Izquierdo A, Lazo L, Marcos E, Guzmán G, Muzio V, L. Herrera. Virus-like particles as nanovaccine candidates. 2013. Adv. Nat. Sci.: Nanosci. Nanotechnol. 4(1).

131. Iris Valdés, Lisset Hermida, Lázaro Gil, Laura Lazo, Gerardo Guillén, María G Guzmán, Jorge Castro, Yaremis Romero, Pedro Puente, Jorge Martín, Ernesto Marcos, Carlos López, Jorge Sánchez, Olivia Niebla, Tamara Menéndez, Alienys Izquierdo, Edith Suzarte, José A Silva. Proof of concept in non-human primates of the heterologous prime-boost strategy combining dengue-2 virus and recombinant proteins including domain III of the viral envelope protein. 2013. Biotecnología Aplicada. 30(4): 150-152.

132. Jorge Berlanga-Acosta, Pedro López-Saura, Isabel Guillen-Pérez, Gerardo Guillen-Nieto, Boris Acevedo-Castro and Luis Herrera-Martínez. Type 2 Diabetes Mellitus (T2DM). Biological overview from pathways to organelles and its translation toward a torpid wound healing process. 2013. J Diabetes Metab 4(7): 285. http://dx.doi.org/10.4172/2155-6156.1000285

133 Iris Valdésa, Lázaro Gila, Jorge Castroa, Damian Odoyob, Rikoi Hitlerb, Elephas Muneneb, Yaremis Romeroa, Lucy Ocholab, Karelia Cosmea, Thomas Kariukib, Gerardo Guillén, Lisset Hermidaa Olive baboons: a non-human primate model for testing dengue-2 virus replication. 2013. International Journal of Infectious Diseases. 17 (12): e1176–e1181.

134 Guillén, Isabel A; Berlanga, Jorge; Camacho, Hanlet; Fernández-de-Cossio, María E; Pérez, Lincidio; Novoa, Lidia I; Palenzuela, Daniel O; Díaz, Tamara; Guillén, Gerardo E; Herrera, Luis; Cosme, Karelia; Gorovaya, Laritza; Mendoza, Osmani; Fernández, Julio R; Ancizar, Julio A; Suarez, Jose; Tuero, Ángela D; Ochagavía, María E; Roca, Juan; Gavilondo, Jorge; García del Barco, Diana; Martín, Jorge. Molecular mechanisms involved in the inhibition of tumor cells proliferation exposed to elevated concentrations of the epidermal growth factor. 2013. Biotecnología Aplicada. 30 (3): 223-227.

134 Guillén, Isabel A; Berlanga, Jorge; Camacho, Hanlet; Fernández-de-Cossio, María E; Pérez, Lincidio; Novoa, Lidia I; Palenzuela, Daniel O; Díaz, Tamara; Guillén, Gerardo E; Herrera, Luis; Cosme, Karelia; Gorovaya, Laritza; Mendoza, Osmani; Fernández, Julio R; Ancizar, Julio A; Suarez, Jose; Tuero, Ángela D; Ochagavía, María E; Roca, Juan; Gavilondo, Jorge; García del Barco, Diana; Martín, Jorge. Molecular mechanisms involved in the inhibition of tumor cells proliferation exposed to elevated concentrations of the epidermal growth factor. 2013. Biotecnología Aplicada. 30 (3): 223-227.

135. Valiente J., Garcia del Barco D., Berlanga J., Santana H., Guillen G., Altruda F., Tarone G. and L. Silengo. Demostración del efecto inotrópico del CIGB500 mediante ecocardiografia. 2013. Biotecnología Aplicada. 30 (4): 280-284.

136. Valiente J., Garcia del Barco D., Guillen G., Santana H., Altruda F., Tarone G., Silengo L., J. Berlanga. Cardiotropic effect of GHRP-6: in vivo characterization by echocardiography. 2013. Biotecnología Aplicada. 30 (4): 285-289

137. Alicia Santos, Osvaldo Reyes, Ania Cabrales, Yunier Rodríguez, Haydeé Gerónimo, Hilda E Garay, Celia A Arrieta, Miriam Ojeda, Ana C Machado, José Suarez, Julio A Ancisar, Mariela Vázquez, Gerardo Guillén, Araceli Chico, Miguel Estévez, Alexey Llopiz, Jesús Noda, Aniel Sánchez, Lorenzo Silengo, Fiorella Artruda, Silvio Perea. Identification of the first antagonist peptide that inhibits biological effects of interleukin-15. 2013. Biotecnología Aplicada. 30 (4): 317-319.

138. Yssel Mendoza-Mari, Calixto Valdés-Pérez, Elena Rodríguez-Corrales, José Suárez-Alba, Ariana García-Ojalvo, Diana García del Barco, Gerardo Guillén-Nieto, Luis Herrera-Martínez and Jorge Berlanga-Acosta. Histological and Transcriptional Expression differences between Diabetic Foot and Pressure Ulcers 2013. J Diabetes Metab 4 (8): 296.

139 Camacho H, Fernandez ME, Guillen IA, Perez L, Fernandez JR, Tuero AD, Diaz T, Palenzuela D, Berlanga J, Guillen GE, Herrera L, Novoa LI. Analysis of cell proliferation and gene expression profiles in Epidermal Growth Factor-treated tumor cell lines. 2013. Minerva Biotechnology 25 (1): 43-54.

140 Berlanga-Acosta J, Schultz GS, López-Mola E, Guillen-Nieto G, García-Siverio M, Herrera-Martínez L. Glucose toxic effects on granulation tissue productive cells: the diabetics' impaired healing. 2013. Biomed Res Int.: 1-15.

141. Roland Züst, Ying-Xiu Toh, Iris Valdés, Daniela Cerny, Julia Heinrich, Lisset Hermida, Ernesto Marcos, Gerardo Guillén, Ulrich Kalinke, Pei-Yong Shi, and Katja Fink. Type I IFN signals in macrophages and dendritic cells control dengue virus infection: implications for a new mouse model to test dengue vaccines. 2014. Journal of Virology 88(13):7276-7285.

142. Diógenes Quintana-Vázquez, Edelgis Coizeau, Anabel Alvarez, Maité Delgado, Tania Cárdenas, Yassel Ramos, Glay Chinea, Guy A M Berbers, Gerardo E Guillén. Recombinant hybrid proteins from pertactin type 1 and 2 of Bordetella pertussis are more immunogenic in mice than the original molecules. 2014. Biotecnología Aplicada. 31:33-42

143. Fuentes, Franklin; Junco, Jesús; Calzada, Lesvia; Lopez, Yovisleydis; Pimentel, Eulogio; Basulto Baker, Roberto; Reyes Acosta, Osvaldo; Garay-Pérez, Hilda E; Guillén-Nieto, Gerardo. Effect of a GnRH vaccine formulation on testosterone concentrations and reproduction in adult male rats. 2014. Biotecnología Aplicada. 31(3):222-226.

144. Izquierdo A., García A., Lazo L., Gil L., Marcos E., Alvarez M., Valdés I., Hermida L., Guillén G., Guzmán MG. A tetravalent dengue vaccine containing a mix of domain III-P64k and domain III-capsid proteins induces a protective response in mice. 2014. Arch. Virol. 159(10):2597-604

145. Lázaro Gil, Ernesto Marcos, Alienys Izquierdo, Laura Lazo, Iris Valdés, Peris Ambala, Lucy Ochola, Rikoi Hitler, Edith Suzarte, Mayling Alvarez, Prisilla Kimiti, James Ndung'u, Thomas Kariuki, María Guadalupe Guzmán, Gerardo Guillén, Lisset Hermida. The protein DIIIC-2, aggregated with a specific oligodeoxynucleotide and adjuvanted in alum, protects mice and monkeys against DENV-2. 2014. Immunology and Cell Biology 93(1). 4

146. Lazo L., Izquierdo A., Suzarte E., Gil L., Valdés I., Marcos E., Álvarez ., Romero Y., Guzmán MG., Guillén G., Hermida L. Evaluation in mice of the immunogenicity and protective efficacy of a tetravalent subunit vaccine candidate against dengue virus. 2014. Microbiol. Immunol. 58: 219-226.

147. Gil L., Izquierdo A., Lazo L., Valdés I., Ambala P., Ochola L., Marcos E., Suzarte E., Kariuki T., Guzmán G., Guillén G., Hermida L. Capsid protein: Evidences about the partial protective role of neutralizing antibody-independent immunity against dengue in monkeys. 2014. Virology 456-457: 70–76.

148. Suzarte, E., Marcos, E., Gil, L., Valdés, I., Lazo, L., Ramos, Y., Pérez, Y., Falcón, V., Romero, Y., Guzmán, M.G., González, S., Kourí, J., Guillén, G., Hermida, L. Generation and characterization of potential dengue vaccine candidates based on domain III of the envelope protein and the capsid protein of the four serotypes of dengue virus. 2014. Arch. Virol. 159(7): 1629-1640.

149. Carlos Cabal-Mirabal, Evelio González Dalmau, Jorge Berlanga Acosta, Daniel Darias Zayas, Luis Herrera Martínez, Pedro Lopez Saura, Gerardo Guillén Nieto, Julio Baldomero Hernández, Carlos González Delgado and Luis Javier González. Quantitative studies of the evolution of Diabetic foot lesions under EGF treatment by Magnetic Resonance Imaging. 2014. Journal of Radiology Research and Practice. (2014), Article ID 783980.

150. Diogenes Quintana, Maité Delgado, Gilda Lemos, Edelgis Coizeau, Yassel Ramos, Yanet Támbara, Luis Javier Gonzales, Gerardo E. Guillén, Anabel Álvarez. Assessment of the Bordetella pertussis BpCNIC0311 as producing strain for genetically detoxified toxoid (PTg), filamentous hemagglutinin (FHA) and pertactin type 2 (Prn2)" submitted for publication in the Journal of Infectious Diseases and Therapeutics. 2015. Journal of Infectious Diseases and Therapeutics 3(1):8-20

151. Roland Zuest, Iris Valdes, David Skibinski, Yufang Lin, Bijin Au, Toh Ying Xiu, Thavamalar Balakrishnan, Katherine Chan, Lisset Hermida, John Connolly, Gerardo Guillen and Katja Fink.Tetravalent Dengue DIIIC Protein together with Alum and ODN elicits a Th1 Response and Neutralizing Antibodies in Mice. 2015. Vaccine 33(12): 1474-82.

152. Carmen L. Loureiro, Julio C Aguilar, Jorge Aguiar, Verena Muzio, Eduardo Penton, Daymir Garcia, Gerardo Guillen, Flor H. Pujol. HBV genotyping variability in Cuba. 2015. PloS ONE. 10(3):e)118959. DOI: 10.1371/journal.pone.0118959

153. Alicia Santos Savio, Ana Cecilia Machado Diaz, Araceli Chico Capote, Jamilet Miranda Navarro, Yunier Rodríguez Alvarez, Ricardo Bringas Pérez, Miguel Estévez del Toro and Gerardo E Guillen Nieto. Differential expression of pro-inflammatory cytokines IL-15Ralpha, IL-15, IL-6 and TNFalpha in synovial fluid from Rheumatoid arthritis patients. 2015. BMC Musculoskeletal Disorders 03; 16: 516.

154. Suzarte E, Gil L, Valdés I, Marcos E, Lazo L, Izquierdo A, García A, López L, Álvarez M, Pérez Y, Castro J, Romero Y, Guzmán MG, Guillén G, Hermida L. A novel tetravalent formulation combining the four aggregated domain III-capsid proteins from dengue viruses induces a functional immune response in mice and monkeys. 2015. International Immunology. 27(8):367-79

155. Iris Valdés, Lázaro Gil, Laura Lazo, Ernesto Marcos, Jorge Martín, Edith Suzarte · Jorge Castro, Yaremis Romero, Gerardo Guillén, Lisset Hermida. Could an experimental dengue virus infection fail to induce solid immunity against homologous viral challenge in non-human primates?. 2016. Archives of Virology. 161(2):465-70

156. Healing Enhancement of Diabetic Wounds by Locally Infiltrated Epidermal Growth Factor Is Associated to Systemic Oxidative Stress Reduction. 2016. International Wound Journal. (In press)

157. Jesús A. Junco Barranco, Robert P. Millar, Franklin Fuentes, Eddy Eover, Eulogio Pimentel, Roberto Basulto, Lesvia Calzada, Rolando Morán, Ayni Rodríguez, Hilda Garay, Osvaldo Reyes, Maria D. Castro, Ricardo Bringas, Niurka Arteaga, Henio Toudurí, Mauricio Rabassa, Yairis Fernández, Andrés Serradelo, Eduardo Hernández and Gerardo E. Guillén. Gradual reduction of testosterone using a gonadotropin releasing hormone vaccination delays castration resistance in a prostate cancer model. 2016. Oncology Letters 12: 963-970.

158. Ernesto Marcos, Laura Lazo, Lázaro Gil, Alienys Izquierdo, Edith Suzarte, Iris Valdés, Aracelys Blanco, Julio Ancizar, José Suárez Alba, Yusleydis de la C. Pérez, Karen Cobas, Yaremis Romero, Gerardo Guillén, María G. Guzmán and Lisset Hermida. Dengue encephalitis-associated immunopathology in the mouse model: implications for vaccine developers and antigens inducer of cellular immune response. 2016. Immunology Letters. (In press)

159. Jorge Aguiar, Gerardo García, Yamila León, Eduardo Canales, José Angel Silva, Omar Gell, Regla Estrada, Ivis Morán, Verena Muzio, Gerardo Guillén, Eduardo Pentón, Julio Cesar Aguilar. High Functional Stability of a Low-cost HBV DNA qPCR Primer Pair and Plasmid Standard 2016. EJOHG (In press)

160. Gil, L.,…..A tetravalent formulation based on recombinant nucleocapsid-like particles from dengue viruses induces a functional immune response in mice and monkeys. 2016. J of Immunology (In press)

161. Garcia, A. ….Synthetic growth hormone releasing peptides (GHRPs): a historical appraisal of the evidences supporting their cytoprotective effects. 2016. Clinical Medicine Insights: Cardiology (In press)

162. Loabiana Mato Y., Aguilar Rubido J.C.. Guillen Nieto G.E. HeberNasvac, a novel therapeutic vaccine for chronic hepatitis B patients. 2016. Almanac of Clinical Medicine 44

 

 

PATENTES: 44


OTROS ASPECTOS DE INTERES

Desde el 2005

Secretario del Consejo Científico del CIGB

1995

Miembro del Comité Nacional de Expertos en Vacunas.

2012

Miembro Titular de la Sociedad cubana de Inmunología desde 1996 y Miembro de Honor.

2005

Miembro del Tribunal Permanente de Infectología y Medicina Tropical.

2010

Miembro del Editorial Board de la Revista “Advances in Prevention Medicine”

2012

Miembro del Editorial Board de la Revista Advances in Natural Sciences: Nanoscience and Nanotechnology

2013

Miembro del Consejo Científico de BIOCUBAFARMA

Desde Oct. 2015 al 2018

Miembro del Consejo Científico del Instituto de Medicina Tropical

HONORES PROFESIONALES

1993 – 2015

Autor o coautor de 44 Premios Anuales de la Academia de Ciencias de Cuba por los resultados científicos

1997

Copa por el II lugar en evento EUREKA en Bélgica a la patente sobre el antígeno vacunal P64k.

2004

Premio Anual de la Academia de Ciencias del Tercer Mundo y ACC a Joven Investigador en Ciencias Biológicas

2006

Orden Carlos J. Finlay

2008

Placa Conmemorativa 485 Aniversario de la Villa de San Cristóbal de La Habana, otorgada por los méritos en la investigación científica.

2011

Medalla OMPI a Patente: Uso de una composición farmacéutica que contiene Factor de Crecimiento Epidérmico (EGF) y procedimiento para corregir el microambiente de heridas y úlceras cutáneas crónicas. Premio a la Creatividad y la Innovación Tecnológica 2011, OCPI

TESIS TUTORIADAS

15, de ellas tres Tesis de Grado a Doctor en Ciencias

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